Effect of Astragalus polysaccharide on immune effect of attenuated avian infectious bronchitis vaccine in chickens
The experiment investigated the immunomodulatory effects of APS on the avian infectious bronchitis attenuated vaccine,aiming to explore the potential application of APS as a vaccine adjuvant.A total of 120 fourteen-day-old chickens were randomly divided into three groups with four replicates per group and one chickens per replicate,designated as the APS+H120 co-immunization group,the H120 vaccine group,and the PBS negative control group.All groups of chickens were immunized three times at two-week intervals,with each nasal inoculation consisting of 100 μL per chicken.Fourteen days after the final immunization,the chickens were intranasally challenged with the infectious bronchitis virus strain M41.The clinical symptoms of chickens were observed,and the amount of lung and liver virus was detected.The results showed that compared with the H120 vaccine group,the APS+H120 vaccine combined immunization can induce the production of IBV specific IgG and sIgA in 14-day-old chickens,effectively protecting them against the attack of allogenic strains,and the survival rate of laying hens was 100％.The APS+H120 group did not show the typical clinical symptoms of avian infectious bronchitis,and the production performance of laying hens was not affected.The study indicates that APS has a promoting effect on the immune response of chickens to the infectious bronchitis H120 attenuated vaccine.
万方数据
维普
