The paper aims to develop a method to rapidly detect the content of tylosin tartrate.In Clark-Lubs buffer solution with pH 6.6,malachite green can induce strong resonance light scattering due to the construction of supermolecule aggregates,and tylosin tartrate can react with malachite green cations,resulting in the decomposition of supermolecule aggregates constructed by malachite green,which significantly weakens the resonance light scattering intensity of the system.The maximum detection wavelengths are at 285 nm and 332 nm.Due to the additive nature of the resonance light intensity(I),the two wavelengths are superimposed for measurement.The linear range of tylosin tartrate is 0.2～0.6 mg/L,the linear regression equation is IRLS=5 299C-766.4,the correlation coefficient(R)is 0.999 8,and the detection limit is 0.004 1 mg/L.The result indicates that the method is simple and sensitive,and can be successfully applied for the determination of tylosin tartrate.
关键词
酒石酸泰乐菌素/孔雀石绿/超分子聚集体/双波长叠加/电荷转移反应/共振光散射法
Key words
tylosin tartrate/malachite green supramolecular aggregate/supermolecule aggregates/double wavelength superposition/charge transfer reaction/resonant light scattering
维普
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