首页|孔雀石绿双波长叠加共振光散射光谱法测定酒石酸泰乐菌素含量

孔雀石绿双波长叠加共振光散射光谱法测定酒石酸泰乐菌素含量

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为了快速检测酒石酸泰乐菌素的含量,在pH值6.6 Clark-Lubs缓冲溶液条件下,孔雀石绿因构筑超分子聚集体而产生强力的共振光散射,酒石酸泰乐菌素能够与孔雀石绿阳离子发生反应,导致孔雀石绿所构筑的超分子聚集体分解,明显减弱了体系共振光散射强度,其最大的检测波长位于285 nm和332 nm.由于共振光强度(I)具有加和性,故将两个波长叠加来进行测定.酒石酸泰乐菌素的线性范围为0.2～0.6 mg/L,线性回归方程为IRLs=5 299C-766.4,相关系数(R)为0.999 8,检测限为0.004 1 mg/L.研究表明,所建立的方法具备操作简便和灵敏度高等特点,可用于酒石酸泰乐菌素含量的测定.
Determination of tylosin tartrate by resonance light scattering spectrometry with malachite green dual wavelength superposition
The paper aims to develop a method to rapidly detect the content of tylosin tartrate.In Clark-Lubs buffer solution with pH 6.6,malachite green can induce strong resonance light scattering due to the construction of supermolecule aggregates,and tylosin tartrate can react with malachite green cations,resulting in the decomposition of supermolecule aggregates constructed by malachite green,which significantly weakens the resonance light scattering intensity of the system.The maximum detection wavelengths are at 285 nm and 332 nm.Due to the additive nature of the resonance light intensity(I),the two wavelengths are superimposed for measurement.The linear range of tylosin tartrate is 0.2～0.6 mg/L,the linear regression equation is IRLS=5 299C-766.4,the correlation coefficient(R)is 0.999 8,and the detection limit is 0.004 1 mg/L.The result indicates that the method is simple and sensitive,and can be successfully applied for the determination of tylosin tartrate.

tylosin tartratemalachite green supramolecular aggregatesupermolecule aggregatesdouble wavelength superpositioncharge transfer reactionresonant light scattering

袁莉、雷玉办、莫江敏、韦后明、马卫兴

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广西现代职业技术学院智能冶金学院,广西河池 547000

江苏海洋大学药学院,江苏连云港 222005

酒石酸泰乐菌素 孔雀石绿 超分子聚集体 双波长叠加 电荷转移反应 共振光散射法

2021年江苏省首批一流本科课程2023年度广西高校中青年教师科研基础能力提升项目

2023KY1467

2024

10.13557/j.cnki.issn1002-2813.2024.03.022

饲料研究
北京市营养源研究所

饲料研究

CSTPCD北大核心
影响因子：0.391
ISSN：1002-2813
年,卷(期)：2024.47(3)
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