摘要
试验旨在研究复合益生菌发酵饲料对肉羊生长性能、养分表观消化率、粪便微生物菌群和血清指标的影响.选择64只体重(29.25±0.35)kg、健康的杜寒杂交育肥公羊,随机分为4组,每组4个重复,每个重复4只羊.对照组、试验1组、试验2组、试验3组分别在基础饲粮中添加0、5％、10％、15％复合益生菌发酵饲料.试验期60 d.结果显示,与对照组相比,试验1组平均日增重显著升高(P＜0.05),试验2组和试验3组末重和平均日增重均显著升高(P＜0.05),料重比均显著降低(P＜0.05);试验2组和试验3组的粗蛋白(CP)、粗脂肪(EE)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)表观消化率均显著升高(P＜0.05);试验1组、试验2组和试验3组的乳酸杆菌、双歧杆菌数量均显著升高(P＜0.05),大肠杆菌数量均显著降低(P＜0.05);试验2组和试验3组的沙门氏菌数量显著降低(P＜0.05).与对照组相比,试验2组、试验3组的免疫球蛋白G(IgG)、免疫球蛋白A(IgA)、白细胞介素-2(IL-2)、肿瘤坏死因子-α(TNF-α)、总蛋白(TP)、白蛋白(ALB)含量以及总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性均显著升高(P＜0.05),谷丙转氨酶(ALT)、谷草转氨酶(AST)活性以及尿素氮(BUN)、丙二醛(MDA)含量均显著降低(P＜0.05).研究表明,肉羊饲粮中添加10％和15％复合益生菌发酵饲料能够改善肉羊的生长性能、粪便微生物菌群和血清指标,提高养分表观消化率.
Abstract
The purpose of this experiment was to study the effects of compound probiotic fermented feed on the growth performance,nutrient apparent digestibility,fecal microbial flora,and serum indexes of mutton sheep.Sixty-four(29.25±0.35)kg healthy Du-Han hybrid fattening rams were randomly divided into four groups with four replicates in each group and four sheep in each replicate.The control group,test group 1,test group 2,and test group 3 were fed with 0,5％,10％,and 15％compound probiotic fermented feed,respectively.The trial period was 60 days.The results showed that compared with the control group,the average daily gain of the test group 1 increased significantly(P＜0.05).The final weight and average daily gain of the test group 2 and test group 3 increased significantly(P＜0.05),and the feed-to-weight ratio decreased significantly(P＜0.05).The apparent digestibility of CP,EE,NDF,and ADF in the test group 2 and test group 3 increased significantly(P＜0.05).The number of Lactobacillus and Bifidobacterium in the test group 1,test group 2,and test group 3 increased significantly(P＜0.05),while the number of Escherichia coli decreased significantly(P＜0.05).The number of Salmonella in the test group 2 and test group 3 decreased significantly(P＜0.05).Compared with the control group,the contentrations of IgG,IgA,IL-2,TNF-α,TP,ALB,T-AOC,and SOD activities were significantly increased(P＜0.05),while the activities of ALT,AST,BUN,and MDA were significantly decreased(P＜0.05).The result shows that adding 10％and 15％compound probiotic fermented feed to mutton sheep diet can improve the growth performance,fecal microbial flora and serum indexes of mutton sheep,and increase the apparent digestibility of nutrients.
国家科技期刊平台
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