Effect of artificial culture on intestinal flora of Nile tilapia
The purpose of the experiment was to study the effect of artificial culture on intestinal flora of Nile tilapia.The intestinal contents of wild(YS)and cultured(YZ)tilapia were sequenced by 16S rDNA sequencing method,and the differences of intestinal flora richness and diversity between them were analyzed and compared.The results showed that Actinobacteriota,Proteobacteria and Firmicutes were the main dominant flora in the intestinal tract of Nile tilapia in wild environment.Actinobacteriota,Proteobacteria and Cyanobacteria were the main dominant flora in artificial culture environment.The analysis of intestinal flora diversity showed that the community diversity index in YZ group was lower than that in YS group(P＜0.05).At the phylum level,Proteobacteria,Acidobacteriota,Bacteroidota and NBl-j in YZ group were lower than those in YS group(P＜0.01 or P＜0.05),while Cyanobacteria and Dependentiae were higher than those in YS group(P＜0.01 or P＜0.05).At the genera level,the genera of norank_f norank_o Chloroplast,Mycobacterium,Paeniclostridium,IMCC26207,Methylocystis and Conexibacter in YZ group were higher than those in YS group(P＜0.0l),while the genera of Exiguobacterium,norank_f_Steroidobacteraceae and norank_f_SC-I-84 were lower than those in YS group(P＜0.0l).Spearman correlation analysis showed that the order of environmental factors on the distribution of species data was pH＞NH4-N＞NO2-N＞O＞PO4-P.At the level of genus classification,the influence of environmental factors on the distribution of species data in different grouping samples was in the order of NO2-N＞NH4-N＞pH＞O＞PO4-P.The study indicates that artificial culture will change the intestinal flora of Nile tilapia,which is helpful to explain the effects of intestinal flora on the health and growth of Nile tilapia in cultured and wild environments.
Nile tilapiaintestinal floradifferent culture environments16S rDNA sequence
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