Research on mixed solid-state fermentation of pure flavor distiller's grains for protein feed production
The study utilized pure flavor distiller's grains as the raw material to prepare protein feed through mixed solid-state fermentation.The fermentation involved the addition of brewing yeast(SY),Bacillus subtilis D,and Lactobacillus plantarum 2-41.The research explored the influence of bran adding amount on the quality of the mixed solid-state fermented feed.After optimizing the fermentation medium composition,single-factor experiments and orthogonal experiments were conducted,with crude protein content in the distiller's grains feed as the evaluation indicator.The study investigated the effects of fermentation temperature,fermentation time,inoculum size,and initial acidity of the fermentation medium on the quality of the mixed solid-state fermented feed,aiming to optimize the fermentation process conditions.The results revealed that the optimal distiller's grains to bran ratio in the fermentation medium was 9∶1,with an initial moisture content of 50％～60％,the total loading amount of the fermentation medium was 50 g,including 45 g pure flavor distiller's grains and 5 g bran.The optimal conditions for mixed solid-state fermentation were an inoculum size of 13％,fermentation temperature of 26 ℃,fermentation time of six days,and initial acidity of 0.90 mmol NaOH/10 g.Three parallel experiments were conducted under these optimal conditions,resulting in a crude protein content of(24.97±0.05)％in the distiller's grains feed.The sensory evaluation scored 18 points,with an overall rating of excellent.The study results provide valuable guidance for the mixed solid-state fermentation of distiller's grains to produce protein feed.
pure flavor distiller's grainsmixed bacteria solid state fermentationprotein feedprocess optimization
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