Safety evaluation of a Bacillus amyloliquefaciens form plant endophytic bacterium
The experiment aimed to evaluate the safety of a Bacillus amyloliquefaciens isolated from Eucommia ulmoides.Safety assessments were conducted through comprehensive evaluations of hemolysis,amino acid decarboxylation,gelatin liquefaction tests,antibiotic sensitivity,intraperitoneal injection,oral gavage,and antimicrobial activity.The intraperitoneal injection experiment included an injection group(1.0 × 1010 CFU/0.3 mL)and a normal saline group,with a single injection and continuous observation for five days.The oral gavage experiment comprised a high-dose group(1.0 × 1010 CFU/0.2 mL),a medium-dose group(1.0 × 109 CFU/0.2 mL),a low-dose group(1.0 × 108 CFU/0.2 mL),and a normal saline group,with daily gavage for 28 days.The results showed that both intraperitoneal injection and oral gavage mice exhibited healthy survival,with no clinical changes observed.During the experimental period,the body weight increased normally,and there were no significant differences in blood routine,serum biochemical indicators,and organ indices among the groups(P＞0.05).Bacillus strain DZ01 did not exhibit translocation,and hemolysis,amino acid decarboxylation,and gelatin liquefaction tests were all negative.Bacillus strain DZ01 showed sensitivity to all eight tested antibiotics.Apart from Enterococcus faecalis and Pseudomonas aeruginosa,the supernatant of Bacillus strain DZ01 exhibited inhibitory activity against the other six indicator strains.The study suggests that Bacillus strain DZ01 has good safety.
Bacillus amyloliquefacienssafetyendophytic bacteria in plants
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