Rapid detection of diazepam in aquatic feed based on a direct-pass-through SPE column
The research aims to establish a rapid and accurate method for the determination of diazepam in aquatic feed,based on direct solid-phase extraction(SPE)column combined with ultra-high-performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).After 10 mL of acetonitrile-formic acid water extraction and defatting with isooctane,the sample is shaken,centrifuged,and the upper clear liquid is purified through direct pass-through SPE column,filtered through a 0.22 μm membrane,and then subjected to machine analysis.The results show that the linear coefficient is greater than 0.99,with a detection limit of 0.3 μg/kg and a quantification limit of 1.0 μg/kg.For diazepam added at low,medium,and high levels to blank samples,the recovery rates range from 82.6％to 101.4％.The intra-batch relative standard deviation(RSD)is between 1.8％and 6.2％,and the inter-batch RSD is between 3.4％and 5.7％.The study indicates that this method is time-efficient,cost-effective,and accurate,making it suitable for the determination of diazepam in large-scale aquatic feed samples.
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