The experiment studied the effect of fish oil on lactation performance and growth performance of suckling piglets.In this study,a total of 56 healthy sows in the late gestation period(107 days)were randomly divided into four groups with 14 replicates in each group and one sow per replicate.The control group was fed with corn-soybean meal basic diet+2.0%soybean oil,and the experimental groups were supplemented with 0.5%,1.0%,and 2.0%fish oil,respectively.The test period ranged from 107 days of gestation to 18 days postpartum.The results showed that compared with the control group,the addition of 2.0%fish oil significantly increased the average weaning weight of piglets and the average daily gain of piglets during lactation(P<0.05),but had no significant effect on feed intake,backfat loss,weaning estrus interval and survival rate of piglets during lactation(P>0.05).Compared with the control group,the addition of 1.0%or 2.0%fish oil to the diet changed the fatty acid composition of the milk,the content of C22:5n-3 and C22:6n-3 in colostrum and C20:5n-3,C22:5n-3,and C22:6n-3 in normal milk significantly increased(P<0.05),and the proportion of n-3 polyunsaturated fatty acids(n-3 PUFA)in milk was increased.Compared with the control group,the addition of 2.0%fish oil significantly increased the content of immunoglobulin G(IgG)and immunoglobulin M(IgM)in colostrum and IgG and IgM in the plasma of piglets(P<0.05),and significantly increased the content of inflammatory cytokines interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in the plasma of piglets(P<0.05).The study indicates that the addition of 2.0%fish oil to the sow's diet increases the n-3 PUFA and immunoglobulin content in the sow's milk,and improves the lactation growth performance of piglets by enhancing the piglet's immunity and alleviating the inflammatory response.