To determine content of sanguinarine and chelerythrine in Macleaya cordata injection based on HPLC
The purpose of the trial was to evaluate the quality of Macleaya cordata injection product.In the experiment,high performance liquid chromatography was used to establish a simultaneous method for the determination of sanguinarine and chelryerythrine in Macleaya cordata injection products.Agilent mAbylent mAb was used as acetonitrile-0.1％phosphoric acid for gradient elution at a flow rate of 1.0 mL/min,a column temperature of 30 ℃,a detection wavelength of 270 nm,and an injection volume of 5 μL.The results showed that the HPLC method for the determination of sanguinarine and chelerythrine in Macleaya cordata injection had high specificity,good linear range,and good repeatability.The precision RSDs of sanguinarine and ceracernine were 0.45％and 0.09％,the stability RSDs were 0.60％and 0.39％,respectively,and the recoveries ranged from 99.83％～101.79％and 103.47％～105.56％,respectively.The study indicates that the method is stable and reliable,with high precision and accuracy,and can be used to evaluate the quality of commercially available Macleaya cordata injection.
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