Effects of exogenous salicylic acid on germination and seedling growth of millet under PEG stress
In order to explore the effects of exogenous salicylic acid soaking treatment on millet germination and seedling growth under PEG stress,Jizajinmiao NO.1 millet was used as the test material,and the effects of five salicylic acid concentrations(0,50,100,150,and 200 mg/L)on the seed germination process and seedling growth of millet under 20％PEG treatment were determined by Petri dish filter paper method.The results showed that with the increase of salicylic acid concentration,the germination rate,germination potential,germination index,and vigor index of millet seeds showed a trend of first increasing and then decreasing,and when the concentration of salicylic acid was 150 mg/L,all the indexes reached the maximum,which were 95.33％,90.67％,1.12,and 39.22,respectively,which were 1.22,1.30,1.36,and 2.12 times that of the distilled water control group(the alicylic acid concentration was 0 mg/L).The germination rate,germination potential,germination index,and vigor index of millet seeds under PEG stress were higher than those of the PEG control treatment(the salicylic acid concentration was 0 mg/L and 20％PEG solution was added),the salicylate concentration increased first and then decreased with the increase of salicylate concentration,and the indexes reached the maximum when the salicylic acid concentration was 150 mg/L.With the increase of salicylic acid concentration,root length,plant height,fresh weight,chlorophyll content,proline content,and peroxidase(POD)activity of foxtail millet seedlings increased first and then decreased,while the relative conductivity and malondialdehyde(MDA)content showed a trend of first decreasing and then increasing.The results showed that the salicylic acid had a significant effect on seed germination and seedling growth of millet under drought stress,and the better treatment effect was achieved when the concentration of salicylic acid was 150 mg/L.
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