首页|灭活植物乳杆菌及其代谢物对克氏原螯虾生长性能和肠道健康的影响

灭活植物乳杆菌及其代谢物对克氏原螯虾生长性能和肠道健康的影响

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  • 国家科技期刊平台
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试验旨在研究灭活植物乳杆菌及其代谢物(LPM)对克氏原螯虾生长性能和肠道健康的影响。试验选择初重为(8。16±0。23)g的克氏原螯虾162尾随机分为3组，每组3个重复，每个重复18尾虾。配制3组分别含0(对照组)、300(LPM-300组)、600 mg/kg(LPM-600组)的LPM的配合饲料。配制两个试验组饲料时，在对照组饲料配方基础上用3％面粉代替3％的花生粕，使饲料蛋白质水平降低1。5％。试验期42 d。结果显示:饲料中添加600 mg/kg LPM，克氏原螯虾的增重率、特定生长率、蛋白质效率和蛋白质沉积率显著高于对照组(P＜0。05)，饲料系数显著低于对照组(P＜0。05)。饲料中添加600 mg/kg LPM，全虾粗蛋白、粗脂肪和粗灰分含量显著高于对照组(P＜0。05)。饲料中添加300、600 mg/kg LPM，肌肉粗脂肪含量显著高于对照组(P＜0。05)。与对照组相比，饲料中添加LPM可显著提高克氏原螯虾血清总蛋白(TP)含量(P＜0。05)，降低谷草转氨酶(AST)和谷丙转氨酶(ALT)活性(P＜0。05);饲料中添加600 mg/kg LPM可显著提高克氏原螯虾血清白蛋白(ALB)含量和碱性磷酸酶(AKP)活性(P＜0。05)。添加600 mg/kg LPM可显著提高肝胰脏和肠道蛋白酶活性(P＜0。05)。添加600 mg/kg LPM可显著降低克氏原螯虾绒毛宽度(P＜0。05)。对照组和试验组的肝胰脏细胞结构完整，无损伤或病变，LPM-300组的B细胞、F细胞和R细胞数量明显多于对照组。研究表明，饲料中添加600 mg/kg LPM有利于改善克氏原螯虾肠道组织结构，提高非特异性免疫能力，促进了饲料蛋白质的利用。
Effects of inactivated Lactobacillus plantarum and its metabolites on growth performance and intestinal health of Procambarus clarkii
The purpose of this study was to investigate the effects of inactivated Lactobacillus plantarum and its metabolite(LPM)on the growth performance and intestinal health of Procambarus clarkii.A total of 162 Procambarus clarkii with an initial weight of(8.16±0.23)g were randomly divided into three groups with three replicates in each group and 18 shrimps in each replicate.Three groups of compound diets containing 0(control group),300(LPM-300 group),600 mg/kg(LPM-600 group)of Lactobacillus plantarum and its metabolite(LPM)were prepared.In the two experimental groups,3％flour was used instead of 3％peanut meal on the basis of the feed formula of the control group,which reduced the protein level of the feed by 1.5％.The trial period was 42 days.The results showed that the weight gain rate,specific growth rate,protein efficiency and protein deposition rate of Procambarus clarkii were significantly higher than those of the control group(P＜0.05),while the feed coefficient was significantly lower than that of the control group when 600 mg/kg LPM was added to the diet(P＜0.05).The contentations of crude protein,crude fat and crude ash of whole shrimp fed with 600 mg/kg LPM were significantly higher than that of the control group(P＜0.05).When 300 and 600 mg/kg LPM was added to the diet,the crude fat content of muscle was significantly higher than that of the control group(P＜0.05).Compared with the control group,the content of serum total protein(TP)increased significantly(P＜0.05),and the activities of aspartate-oxaloacetic transaminase(AST)and alanine-pyruvic transaminase(ALT)decreased when LPM was added to the diet(P＜0.05).The content of serum albumin(ALB)and the activity of alkaline phosphatase(AKP)were significantly increased when 600 mg/kg LPM was added to the diet(P＜0.05).The protease activity of hepatopancreas and intestinal tract was significantly increased by adding 600 mg/kg LPM(P＜0.05).The addition of 600 mg/kg LPM significantly reduced the villus width of the shrimp compared with the control group(P＜0.05).The hepatopancreas cells in the control group and the experimental group were intact without damage or pathological changes.The number of B cells,F cells,and R cells in the LPM-300 group was significantly more than that in the control group.The study indicates that the addition of 600 mg/kg LPM in the feed can improve the intestinal tissue structure of Procambarus clarkii,enhance the non-specific immunity and promote the utilization of feed protein.

Procambarus clarkiiinactivated Lactobacillus plantarum and its metabolitesgrowth performanceintestinal health

石英、庞纪彩、田娟、刘晶晶、高欣、宋晓青

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山东畜牧兽医职业学院,山东潍坊 261061

中国水产科学研究院长江水产研究所,湖北武汉 430223

江西好实沃生物技术有限公司,江西宜春 336000

克氏原螯虾 灭活植物乳杆菌及其代谢物 生长性能 肠道健康

中国水产科学研究院基本科研业务费专项山东畜牧兽医职业学院科技发展计划山东畜牧兽医职业学院科技发展计划

2022XT012022zrkxzd0022022370701128088

2024

10.13557/j.cnki.issn1002-2813.2024.07.010

饲料研究
北京市营养源研究所

饲料研究

CSTPCD北大核心
影响因子：0.391
ISSN：1002-2813
年,卷(期)：2024.47(7)
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