Captiva EMR-Lipid SPE/ultra performance liquid chromatography-tandem mass spectrometry rapid screening seven mycotoxins in feed
The aim of this study was to rapidly screen seven mycotoxins in feed using Captiva EMR-Lipid solid phase extraction technology combined with ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The prepared feed samples were extracted by ultrasonic(combined with shaking)in 84％acetonitrile aqueous solution for 10 min,centrifuged and purified by one-step pass-through degreasing on a Captiva EMR-Lipid solid-phase extraction column.Then UPLC-MS/MS Electrospray Ionization(ESI),positive and negative ion scanning,multi-reflection monitoring mode(MRM),and matrix-matched external standard quantification were carried out.The results showed that the extraction,purification and detection of seven mycotoxins in a single feed sample could be completed within 30 min,the correlation coefficient(r)of the matrix matching standard curve was greater than 0.994,the detection limit was 2～10 μg/kg,the average recovery rate was 61.9％～123.5％,and the relative standard deviation(RSD)was ≤9.2％(n=5).The study indicated that the method had few pretreatment steps,was easy to operate,the screening was qualitatively accurate,and the detection and analysis throughput was relatively high,which met the screening and analysis of seven mycotoxins in feed.
ultra-performance liquid chromatography-tandem mass spectrometryfeedmycotoxins
国家科技期刊平台
NSTL
万方数据
维普
