牛大力多糖的荧光标记、鉴定及其体内分布
Fluorescence labeling,identification,and in vivo distribution of Millettia speciosa polysaccharide
多水银 1张兴波 1吴玉辉 1李静涛 1杨雨辉1
作者信息
- 1. 海南大学热带农林学院,海南海口 570100
- 折叠
摘要
研究旨在探究牛大力多糖在小鼠体内的吸收与分布.使用罗丹明B标记牛大力多糖得到牛大力多糖罗丹明B荧光标记物(MSP-RhB).使用荧光显微镜和红外光谱扫描鉴定标记成功后,给小鼠灌胃MSP-RhB,分别于1、3、6、12、24 h取材,通过小动物活体成像仪系统观察MSP-Rh B在小鼠体内、体外的分布情况.结果显示:荧光标记牛大力多糖成功;灌胃小鼠后,MSP-Rh B主要分布于胃肠道、肝脏和肾脏;MSP-RhB在1h到达小肠各段,约3 h达到最大值,在小肠中停留时间长达24 h.研究表明,MSP-Rh B通过小肠吸收,先进入全身循环,然后分布到肝脏和肾脏中,且荧光信号随时间的推移逐渐减弱.
Abstract
The study was to explore the absorption and distribution of Millettia speciosa polysaccharide in mice.The Millettia speciosa polysaccharide Rhodamine B fluorescent marker(MSP-Rh B)was obtained by labeling Millettia speciosa polysaccharide with Rhodamine B.After the labels were identified by fluorescence microscope and infrared spectrum scanning,MSP-Rh B was given to mice by intragastric administration.Samples were collected at 1,3,6,12,24 h,respectively,and the distribution of MSP-Rh B in vivo and in vitro was observed by small animal in vivo imaging system.The results showed that fluorescence labeling of Millettia speciosa polysaccharide was successful.After intragastric administration,MSP-Rh B was mainly distributed in gastrointestinal tract,liver and kidney.MSP-Rh B reached each segment of the small intestine at 1 h,reached its maximum at about 3 h,and stayed in the small intestine for 24 h.Studies have shown that MSP-Rh B is absorbed through the small intestine,enters the systemic circulation first,and then distributes to the liver and kidney,and the fluorescence signal gradually decreases with time.
关键词
牛大力多糖/荧光标记/口服吸收/体内分布Key words
Millettia speciosa polysaccharide/fluorescence labeling/oral absorption/in vivo distribution引用本文复制引用
基金项目
国家自然科学基金(32260900)
海南省自然科学基金高层次人才项目(321RC481)
出版年
2024
国家科技期刊平台
NSTL
万方数据