响应面法优化千里光中绿原酸提取工艺
Optimization of extraction process of chlorogenic acid from Senecio scandens Buch-Ham.by response surface methodology
杨凡 1吴静 2刘厚权 3顾震 2谢传奇 2吴磊1
作者信息
- 1. 江西省科学院应用化学研究所,江西南昌 330096;江西农业大学食品科学与工程学院,江西南昌 330045
- 2. 江西省科学院应用化学研究所,江西南昌 330096
- 3. 江西普正制药股份有限公司,江西吉安 331409
- 折叠
摘要
研究采用超声波辅助乙醇提取法从千里光中提取绿原酸,以绿原酸提取率为响应值,在单因素试验考察乙醇浓度、提取温度、提取时间和液料比对千里光绿原酸提取率的影响基础上,借助响应面法优化千里光绿原酸提取的工艺参数.结果显示,超声波辅助乙醇提取千里光绿原酸的优化条件为乙醇浓度50％、提取温度47 ℃、提取时间50 min、液料比20 mL/g.在此条件下,千里光绿原酸提取率可达到1.01％.研究表明,试验确定的提取工艺简单高效,可为千里光绿原酸进一步纯化提取提供参考.
Abstract
In order to study the extraction process by ethanol with the ultrasonic-assisted method of chlorogenic acid in Senecio scandens Buch-Ham.With the content of chlorogenic acid as the index,the effects of ethanol concentration,ultrasonic extraction temperature,extraction time,and material liquid ratio on the extraction rate of chlorogenic acid from Senecio scandens Buch-Ham.were investigated through a single factor experiment.On this basis,the process parameters of chlorogenic acid extraction were optimized by response surface methodology.The results showed that the optimal process parameters were as follows:Ethanol concentration of 50％,ultrasonic temperature of 47 ℃,extraction time of 50 min,and liquid material ratio of 20 mL/g.Under these conditions,the extraction rate of chlorogenic acid could reach 1.01％.The study shows that the extraction process determined by the experiment is simple and efficient,which can provide a reference for further purification and extraction of chlorogenic acid in Senecio scandens Buch-Ham.
关键词
千里光/绿原酸/超声波辅助提取法/响应面法Key words
Senecio scandens Buch-Ham./chlorogenic acid/ultrasonic-assisted method/response surface method引用本文复制引用
基金项目
江西省重点研发计划(20233AAF02063)
江西省科学院省级财政科研项目经费包干制试点示范项目(2023YSBG21021)
江西省科学院省级财政科研项目经费包干制试点示范项目(2021YSBG21016)
出版年
2024
国家科技期刊平台
NSTL
万方数据