黑曲霉固态发酵稻谷壳粉制备木聚糖酶活力饲料辅料研究
Preparation of xylanase active feed auxiliary material by solid-state fermentation of rice hull powder by Aspergillus niger
冯庆玲 1黄宝梅 1谢君铨 1林继辉 1谢勇武1
作者信息
- 1. 闽南科技学院生命科学与化学学院,福建南安 362311
- 折叠
摘要
试验旨在获取具备木聚糖酶活性的发酵稻谷壳粉,以提高稻谷壳粉的饲用价值.试验用黑曲霉对稻谷壳粉进行固态发酵,以发酵产物中木聚糖酶活力为指标,采用单因素结合响应面法优化发酵条件并测量发酵产物营养物质的含量.结果显示,发酵基质质量为10g、初始含水量为10.1 mL、添加1.0％的硫酸铵、培养温度为30.9℃、培养时间为3.1 d时,发酵产物中木聚糖酶活力最高,为135.643 U/mL,较优化前提高了 3.6倍.在此条件下,发酵稻谷壳粉中粗蛋白和真蛋白含量均有提高,真蛋白的含量达12.35％,较发酵前提高了 107.21％,粗纤维和粗脂肪含量有所降低,粗灰分含量提高.研究表明,发酵稻谷壳粉中木聚糖酶活力达到饲用标准,提高了蛋白质含量,可以作为辅料制备饲料.
Abstract
The purpose of the experiment was to obtain fermented rice husk powder with xylanase activity,so as to improve the feeding value of rice husk powder.In this experiment,rice husk powder was fermented by Aspergillus Niger in solid state.The xylanase activity in fermented products was used as an index,and the fermentation conditions were optimized by single factor combined with response surface methodology,and the nutrient content of fermented products was measured.The results showed that when the substrate mass was 10 g,the initial water content was 10.1 mL,1.0％ammonium sulfate was added,the culture temperature was 30.9 ℃,and the culture time was 3.1 d,the highest xylanase activity was 135.643 U/mL,which was 3.6 times higher than that before optimization.Under these conditions,the content of crude protein and true protein in fermented rice husk powder increased,the content of true protein reached 12.35％,which increased by 107.21％compared with that before fermentation,the content of crude fiber and crude fat decreased,and the content of crude ash increased.The study indicates that the xylanase activity in fermented rice husk powder reached the feeding standard,and the protein content is increased,which can be used as an auxiliary material to prepare feed.
关键词
黑曲霉/稻谷壳粉/木聚糖酶活力/饲料辅料Key words
Aspergillus niger,rice husk powder/xylanase activity/feed auxiliary material引用本文复制引用
基金项目
教育厅中青年科技项目(JAT210618)
省级一流本科专业建设点项目(SJZY-2020-02)
出版年
2024
国家科技期刊平台
NSTL
万方数据