Screening and characterization of bacteriostatic Bacillus
The purpose of the experiment was to screen beneficial strains with antibacterial effects from the natural environment and study their growth characteristics.In the experiment,12 strains of Bacillus were screened with E.coli as indicator bacteria,and six strains of Bacillus with antibacterial activity were screened and identified.Then,Escherichia coli and Staphylococcus aureus were used as indicator bacteria to screen the antibacterial peptide extracts of Bacillus,and hemolytic comparison,drug sensitivity determination,and growth characteristics of the screened strains were studied.The results showed that Bacillus G02 and J12,which had strong antibacterial activity,were identified as Bacillus siamensis.The extracts of two strains of Bacillus had the ability of secreting surface activity,and the ability of secreting surface activity was equal.The sensitivity of two strains of Bacillus to 16 drugs was above medium sensitivity.The extracts of two strains of Bacillus had the ability of secreting surface activity,and the ability of secreting surface activity was equal.The sensitivity of two strains of Bacillus to 16 drugs was above medium sensitivity.The two strains entered the logarithmic growth phase at 6 h,the pH value was maintained between 5 and 8,and the antimicrobial activity reached its peak at 22 h.Studies have shown that Bacillus has the ability to produce a variety of active peptides,and has a certain inhibitory effect on common pathogenic bacteria,which is a potential substitute for traditional antibiotics.
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