首页|响应面法优化提取壮药三七姜总酚酸及其镇痛抗炎作用机制研究

响应面法优化提取壮药三七姜总酚酸及其镇痛抗炎作用机制研究

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试验采用响应面法优化壮药三七姜总酚酸提取工艺并探究其镇痛抗炎作用机制.以三七姜为原料,采用回流提取法提取总酚酸,通过单因素试验和Box-Behnken试验对提取工艺进行优化,探究乙醇浓度、液料比、提取时间对总酚酸得率的影响;建立醋酸致小鼠扭体模型和小鼠棉球肉芽肿模型,探究三七姜总酚酸的镇痛抗炎作用机制.结果表明,三七姜总酚酸的最优提取工艺为乙醇浓度42％、液料比60mL/g、回流时间60 min.在此条件下,三七姜总酚酸得率可达6.09 mg/g.与模型组相比,三七姜醇提物各剂量对肉芽肿炎症具有抑制作用,可以降低小鼠扭体次数,降低白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、前列腺素E2(PGE2)、白细胞介素-1β(IL-1β)、5-羟色胺(5-HT)含量,增加环磷酸腺苷(cAMP)含量.研究表明,响应面法优化壮药三七姜总酚酸的提取工艺稳定可靠;三七姜总酚酸具有一定的镇痛抗炎活性,其机制可能与减少体内IL-6、TNF-α、IL-1β和PGE2等促炎性细胞因子分泌有关.
Optimization of response surface method for the extraction of Zhuang medicine Stahlianthus involucratus total phenolic acids and its analgesic and anti-inflammatory mechanism
Response surface method(RSM)was used to optimize the extraction process of Stahlianthus involucratus total phenolic acids and explore its analgesic and anti-inflammatory mechanism.Total phenolic acids were extracted from Stahlianthus involucratus by reflux extraction.The extraction process was optimized by single factor test and Box-Behnken test,and the effects of ethanol concentration,liquid-to-material ratio,and extraction time on the yield of total phenolic acids were explored.Writhing model and cotton ball granuloma model of mice induced by acetic acid were established to study the analgesic and anti-inflammatory mechanism of total phenolic acids in Stahlianthus involucratus.The results showed that the optimum extraction process of total phenolic acids from Stahlianthus involucratus were an ethanol concentration of 42％,liquid-material ratio of 60 mL/g,and reflux time of 60 min.Under these conditions,the yield of total phenolic acids of Stahlianthus involucratus can reach 6.09 mg/g.Compared with the model group,each dose of ethanol extract of Stahlianthus involucratus had an inhibitory effect on granuloma inflammation,reduced the number of writhing,decreased the contents of interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),prostaglandin E2(PGE2),interleukin-1β(IL-1β),5-hydroxytryptamine(5-HT)and increased the content of cyclic adenosine monophosphate(cAMP).The study indicates that the optimized extraction process of Stahlianthus involucratus by response surface methodology is stable and reliable,and can be used for the extraction of total phenolic acids from Stahlianthus involucratus.Stahlianthus involucratus has certain analgesic and anti-inflammatory activities,and its mechanism may be related to reducing the secrete of pro-inflammatory cytokines such as IL-6,TNF-α,IL-1β,and PGE2 in vivo.

Stahlianthus involucratusresponse surface methodtotal phenolic acidsanalgesicanti-inflammation

刘星晨、梁洁、马丽娟、曹玉嫔、陈嘉怡、陈晓思、魏江存、赵立春

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广西中医药大学药学院,广西南宁 530200

广西壮瑶药重点实验室,广西南宁 530200

广西高校中药提取纯化与质量分析重点实验室,广西南宁 530200

壮瑶药协同创新中心,广西南宁 530200

广西壮族自治区民族药资源与应用工程研究中心,广西南宁 530200

广西国际壮医医院,广西南宁 530201

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三七姜 响应面法 总酚酸 镇痛 抗炎

广西自然科学基金国家中医药局高水平中医药重点学科建设项目广西中医药重点学科壮药学项目广西一流学科建设项目(中药学)广西壮族自治区高等学校千名中青年骨干教师培育计划广西中医药大学大学生创新创业训练计划广西中医药大学"岐黄工程"高层次人才团队培育项目(第三批)国家青年岐黄学者项目

2020GXNSFAA238026zyyzdxk-2023165GZXK-Z-20-64桂教科研[2018]12号桂教人[2019]5号2023106001562024062020-7

2024

10.13557/j.cnki.issn1002-2813.2024.09.019

饲料研究
北京市营养源研究所

饲料研究

CSTPCD北大核心
影响因子：0.391
ISSN：1002-2813
年,卷(期)：2024.47(9)
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