Effects of quercetagetin on antioxidant capacity and immune function of broilers under oxidative stress
The aim of this experiment was to investigate the effects of diet supplemented with quercetagetin(QG)on the antioxidant capacity and immune function of broiler chickens under diquat-induced oxidative stress.Two hundred and forty 1-day-old Cobb broilers were selected and randomly divided into a control group,a diquat induced oxidative stress(DOS)group,and a QG group,with eight replicates in each group and 10 broilers in each replicate.The control group and DOS group were fed a basal diet,and the QG group was fed a basal diet supplemented with 100 mg/kg QG.The experiment period was 23 d.One broiler close to the average body weight was selected from each replicate on the 20th day of the experiment.The control group was injected intraperitoneally with 1 mL of saline,and the DOS group and QG group were injected intraperitoneally with the same volume of diquat at a concentration of 20 mg/kg BW.The results showed that compared with the control group,the rate of body weight gain and bursal index in the DOS group significantly decreased(P＜0.05),the concentrations of immunoglobulin A(IgA),immunoglobulin M(IgM),immunoglobulin G(IgG),and complement 4(C4)in the serum of the DOS group were significantly reduced(P＜0.05),the glutathione peroxidase(GSH-Px)activity and expression of GSH-Px mRNA in the liver of boilers in the DOS group were significantly reduced(P＜0.05),and malondialdehyde(MDA)content was significantly increased in the DOS group(P＜0.05).Compared with the DOS group,the rate of body weight gain and bursal index in the QG group were significantly increased(P＜0.05),the concentrations of immunoglobulin A(IgA),immunoglobulin G(IgG),and complement 3(C3)in the serum of the QG group significantly increased(P＜0.05),and the MDA content was significantly reduced in the liver of boilers in the QG group(P＜0.05).The study indicates that adding 100 mg/kg QG to broiler feed can alleviate the weight loss in broilers caused by oxidative stress,by modulating the Nrf2 signaling pathway to improve antioxidant capacity and enhance immune function.
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