Optimization of extraction technology,content determination,and antioxidant activity of total flavonoids from Camellia oleifera cake
The purpose of this study was to optimize the ultrasonic extraction technology of total flavonoids from Camellia oleifera cake by response surface methodology,and to compare the content of total flavonoids from Camellia oleifera cake from different habitats and its antioxidant activity in vitro.Based on the results of single factor experiment,the extraction process of total flavonoids from Camellia oleifera cake was optimized by Box-Behnken response surface methodology with ethanol concentration,ratio of liquid to material,ultrasonic time and ultrasonic power as influencing factors and the yield of total flavonoids as evaluation index,and the extraction process of total flavonoids from Camellia oleifera cake was optimized by using Box-Behnken response surface methodology.The results showed that ethanol concentration of 70％,liquid-material ratio 55 mL/g,ultrasonic time of 45 min,power 500 W were the optimum conditions for ultrasonic extraction of total flavonoids from Camellia oleifera cake.Under these conditions,the actual yield of total flavonoids from Camellia oleifera cake in Nanning,Guangxi was 3.44％.The content of total flavonoids in Camellia oleifera cake from different producing areas and batches ranged from 1.20％to 3.54％,among which Xiangtan City of Hu'nan Province had the highest content of total flavonoids,while Meizhou City of Guangdong Province had the lowest content of total flavonoids.The scavenging ability of DPPH radical of Camellia oleifera cake in Xiangtan City of Hu'nan Province was the highest,and its IC50 value was 0.59 g/L.The free radical scavenging ability of ABTS+was the highest,and its IC50 value was 0.73 g/L.The study indicates that the research can provide references for exploring the exploitation and utilization of Camellia oleifera cake in livestock and poultry breeding.
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