Screening and integration of ALVE and its impact on production performance of Bashang Longtail chickens
The experiment aims to study the distribution of avian leukosis virus(ALVE)in Bashang Longtail chickens and its impact on their production performance.The molecular evolutionary genetics analysis(MEGA)software was used to construct a phylogenetic tree of avian leukosis virus(ALV)using the Neighbor-Joining method,and the Maximum Likelihood method was employed to calculate genetic distances between sequences.Polymerase chain reaction(PCR)was utilized to detect the integration of ALVE in Bashang Longtail chickens,and a two-way ANOVA was conducted to analyze the relationship between the integration of ALVE-6 and ALVE-B5 with egg production and body weight in Bashang Longtail chickens.A paired Chi-square test was used to analyze the association between the integration of ALVE-6 and the positivity of ALV-p27 antigen.The results showed that 24 leukosis viruses were differentiated into three major branches:ALVE,Exogenous Avian Leukosis Virus(ALVK),ALVE and ALVK recombination(ALVK+E/K),ALVA and ALVE and ALVA recombination(ALVA+E/A).The env gene of the viral genome had the highest differentiation coefficient of 0.641,while the 3'UTR had the lowest differentiation coefficient of 0.050,indicating relative conservation.Four types of ALVE were integrated into the genomes of Bashang Longtail Chickens,with the highest integration rate for ALVE-6 at 87.63％.Compared to ALVE-6 negative chickens,the body weight of ALVE-6 positive Bashang Longtail chickens increased significantly(P＜0.05).The PCR detection results for ALVE-6 integration were independent of the ELISA detection results for ALV-p27 antigen.The study indicates that Bashang Longtail chickens have a high rate of ALVE-6 integration,which enhances their production performance,and that the integration of ALVE-6 does not affect the detection results of ALV-p27 antigen.The findings provide support for the application of ALVE in the breeding of Bashang Longtail chickens with excellent production performance.
国家科技期刊平台
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