Application of flocculation technology for solid-liquid separation of Pichia pastoris fermentation broth
The experiment aims to use flocculation technology for solid-liquid separation of the fermentation broth of Pichia pastoris.With the flocculated cell rate and enzyme activity recovery rate as the measurement indicators,single-factor experiments and orthogonal experiments were conducted to study the effects of different flocculants on the flocculation of recombinant Pichia pastoris expressing glucose oxidase in the fermentation broth.The results showed that the optimal flocculation process for the Pichia pastoris fermentation broth was to first add 2.0％polyaluminum chloride,then 1.0％diatomite,and finally 0.2％cationic polyacrylamide,ensuring that the volume of polyaluminum chloride mother liquor added did not exceed 20％of the total volume of the fermentation broth.Under these conditions,the fermentation broth's cell flocculation rate reached over 90％,and the recovery rate of glucose oxidase enzyme activity reached over 85％.The study indicates that the use of flocculation technology can achieve solid-liquid separation of the Pichia pastoris fermentation broth,a method that is beneficial for improving the production efficiency of enzymes,thereby promoting the development of the feed enzyme preparation industry.
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