抗菌肽CC34对小鼠肠炎的缓解作用
Alleviating effect of antimicrobial peptide CC34 on mouse enteritis
董立强 1李云鹤 2张春华2
作者信息
- 1. 绥化学院,黑龙江绥化 152061;黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319
- 2. 绥化学院,黑龙江绥化 152061
- 折叠
摘要
为了揭示抗菌肽CC34对脂多糖(LPS)诱导小鼠肠道炎症的缓解作用及机制,试验选择40只无特定病原体(SPF)级小鼠,随机分为4组,每组10只.CC34组和LPS+CC34组小鼠腹腔注射9 mg/kg CC34,LPS组和Control组小鼠注射等体积生理盐水,持续7d;第7d给药1h后,LPS组、LPS+CC34组小鼠注射7.5 mg/kg LPS,CC34组和Control组注射等体积生理盐水.结果显示,与Control组相比,CC34组小鼠的肝脏、脾脏、心脏指数无显著变化(P＞0.05),但结肠长度极显著增长(P＜0.01).与LPS组相比,LPS+CC34组小鼠肝脏中的荷菌数及十二指肠和回肠中炎性因子的含量极显著降低(P＜0.01);肠道组织中闭锁小带-1(ZO-1)、封闭蛋白-1(Claudin-1)和闭合蛋白(Occludin)的基因表达水平极显著升高(P＜0.01),十二指肠的绒毛高度/隐窝深度值(V/C)显著升高(P＜0.05);肠道组织中髓过氧化物酶(MPO)的基因表达水平极显著降低(P＜0.01);肠组织中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)基因的表达及活性极显著升高(P＜0.01),丙二醛(MDA)含量极显著降低(P＜0.01),总抗氧化能力(T-AOC)极显著增强(P＜0.01);肠组织中Toll样受体-4(TLR-4)和髓样分化因子88(MyD88)的基因表达水平极显著下调(P＜0.01),十二指肠中p65磷酸化水平极显著降低(P＜0.01).研究表明,CC34通过下调TLR-4/核因子κ(NF-κB)信号通路,缓解了小鼠肠道炎症.
Abstract
To reveal the alleviating effect and mechanism of antimicrobial peptide CC34 on lipopolysaccharide(LPS)-induced intestinal inflammation in mice,the experiment selected 40 SPF mice,randomly divided into four groups,with ten mice in each group.Mice in the CC34 group and the LPS+CC34 group were intraperitoneally injected with 9 mg/kg CC34,while mice in the LPS group and Control group were injected with an equal volume of saline,for a duration of seven days.The mice in the LPS group and LPS+CC34 group were injected with 7.5 mg/kg LPS,the CC34 group and Control group injected with equal volume normal saline.The results showed that compared to the Control group,there were no significant changes in the liver,spleen,and heart indices of mice in the CC34 group(P＞0.05),but the colon length extremely increased(P＜0.01).Compared to the LPS group,the bacterial load in the liver and the content of inflammatory factors in the duodenum and ileum of mice in the LPS+CC34 group extremely decreased(P＜0.01),and the gene expression levels of ZO-1,Claudin-1,and Occludin in the intestinal tissue extremely increased(P＜0.01),and the villus height/crypt depth ratio(V/C)in the duodenum significantly increased(P＜0.05).The gene expression level of myeloperoxidase(MPO)in the intestinal tissue extremely decreased(P＜0.01).The gene expression and activity of superoxide dismutase(SOD)and catalase(CAT)in the intestinal tissue extremely increased(P＜0.01),the content of malondialdehyde(MDA)extremely decreased(P＜0.01),and the total antioxidant capacity(T-AOC)extremely enhanced(P＜0.01).The gene expression levels of toll-like receptor-4(TLR-4)and myeloid differentiation factor 88(MyD88)in the intestinal tissue extremely down-regulated(P＜0.01),and the phosphorylation level of p65 in the duodenum extremely decreased(P＜0.01).The study indicates that CC34 alleviates intestinal inflammation in mice by down-regulating the TLR-4/NF-κB signaling pathway.
关键词
抗菌肽/小鼠/脂多糖/肠道炎症/TLR-4/NF-κBKey words
antimicrobial peptide/mouse/lipopolysaccharide/intestinal inflammation/TLR-4/NF-κB引用本文复制引用
基金项目
黑龙江省大学生创新创业培养计划(shxy202350)
黑龙江省省属本科高校基础科研业务费科研项目(YWF10232330202)
黑龙江省自然科学基金(LH2023H055)
药食同源特色产品开发与应用创新团队(230107100208)
出版年
2024
国家科技期刊平台
NSTL
万方数据