Effects of dietary protein levels on growth performance and digestive enzyme activities of Quasipaa spinosa tadpoles
The experiment aimed to investigate the impact of dietary protein levels on the growth performance and digestive enzyme activity of Quasipaa spinosa tadpoles.The composite insect-derived protein was made by mixing mealworm powder,silkworm pupae powder,and fly larvae powder in a 1∶1∶1 ratio,serving as the primary protein source in the feed.Quasipaa spinosa tadpoles with an average weight of(0.32±0.01)g and at the 25th developmental stage were selected.Earthworm powder was used as a feeding stimulant,and casein was added to prepare the feed with protein content of 20％(Group Al),25％(Group A2),30％(Group A3),35％(Group A4),and 40％(Group A5).The Quasipaa spinosa tadpoles were subjected to a 10-week indoor feeding trial under factory conditions at a breeding base with mountain spring water.The results showed that the tadpoles in Group A4 had the highest average weight gain rate(WGR),specific growth rate(SGR),and feed efficiency(FE).The highest metamorphosis rate was observed in Group A3.The highest survival rate was found in Group Al.The intestinal amylase activity in the tadpoles from Group Al was significantly higher than in the other groups(P＜0.05),and the intestinal trypsin activity in the tadpoles from Group A5 was significantly higher than in the other groups(P＜0.05).Regression analysis of the average weight gain and feed efficiency of the Quasipaa spinosa tadpoles revealed that the maximum weight gain was achieved at a protein level of 36.86％in the feed,and the highest feed efficiency was at a protein level of 37.44％.The study suggests that the protein level in the feed affects the growth indicators,feed efficiency,and digestive enzyme activity of the Quasipaa spinosa tadpoles,and the suitable range of protein content in the compound feed for the tadpoles is between 36.86％and 37.44％.
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