Exploring mechanism of Ganoderma lucidum enhancing chicken immune function based on network pharmacology and molecular docking
To investigate the mechanism by which Ganoderma lucidum enhances the immune function in chickens,genes related to chicken immune function and the main effective components of Ganoderma lucidum targets were obtained through databases such as TCMSP.Visualization analysis of the Ganoderma lucidum-target-immune protein-protein interaction(PPI)network was performed using Cytoscape 3.10.0 software,followed by GO and KEGG pathway enrichment analysis through the David database.Finally,molecular docking was conducted using AutoDock software to verify the binding activity of key effective components with key targets.The results showed that a total of 42 effective components of Ganoderma were identified,including 10 key effective components such as Ganoderma lucidum alcohol B,Ganoderma spore lactone B,and Ganoderma lucidum aldehyde B,along with 332 immune-related genes.The PPI network included seven key targets such as Interleukin-6(IL-6),Interleukin-1 beta(IL-1β),and non-receptor tyrosine kinase(SRC).The GO functional enrichment analysis covered 23 biological processes,22 cellular components,and 23 molecular functions.The KEGG pathway enrichment analysis yielded 11 related signaling pathways.The minimum binding energy of the molecular docking was all less than-20.9 kJ/mol,indicating good docking results.The study suggests that the key effective components of Ganoderma that enhance chicken immune function are 10,the key targets are 7,and the related signaling pathways are 11,the results can provide a reference for the application of Ganoderma as a feed additive.
国家科技期刊平台
NSTL
万方数据
