常压蒸馏法提取迷迭香精油的工艺筛选
Study on optimization of extraction conditions of rosemary essential oil by atmospheric distillation
吴维坚 1林洪涛 1杨敏 1王国鑫 2李珊珊1
作者信息
- 1. 福建省农业科学院亚热带农业研究所,福建漳州 363005
- 2. 南靖县植保植检站,福建漳州 363600
- 折叠
摘要
为探索迷迭香精油常压蒸馏工艺的优选方案,试验分别采用不同蒸馏时间、液料比及采样时间对迷迭香鲜叶以水蒸气蒸馏法回流提取,采用顶空进样GC-MS对其中的挥发性成分进行分析比对,利用响应面法优化提取工艺,确定最佳工艺条件.所有试验重复3次.结果显示,各因素对迷迭香精油得率的影响顺序依次为液料比＞采样时间＞蒸馏时间.最佳理论提取条件为蒸馏时长3.96 h、液料比1.50 mL/g、采样时间13:12,迷迭香精油得率理论预测值约为1.551％.验证试验结果表明,采样时间13:10、液料比1.50 mL/g、蒸馏4h,迷迭香精油得率最高,为1.543％,与预测值1.551％存在0.52％的相对误差,其中特征性成分相对含量符合国际标准.研究表明,常压蒸馏提取迷迭香精油的优化工艺方案稳定、有效.
Abstract
To explore the optimal extraction process of rosemary essential oil by atmospheric distillation,the experiment was conducted using different distillation times,liquid-solid material ratios,and sampling times for the hydrodistillation of fresh rosemary leaves with reflux extraction by steam distillation.The volatile components were analyzed and compared using headspace injection GC-MS.The extraction process was optimized using the response surface method to determine the best process conditions.All experiments were repeated three times.The results showed that the order of influence of various factors on the yield of rosemary essential oil was liquid-solid material ratio＞sampling time＞distillation time.The theoretical optimal extraction conditions were determined to be a distillation time of 3.96 hours,a liquid-solid material ratio of 1.50 mL/g,and a sampling time of 13:12,with a theoretical predicted yield of rosemary essential oil of approximately 1.551％.The results showed that the highest yield of rosemary essential oil was 1.543％after sampling time 13:10,liquid-solid ratio 1.50 mL/g and distillation for 4 h,with a relative error of 0.52％compared to the predicted value of 1.551％.The relative content of characteristic components met international standards.The study indicates that the atmospheric distillation extraction process of rosemary essential oil is stable and effective.
关键词
迷迭香精油/提取工艺/常压蒸馏法/GC-MS分析Key words
rosemary essential oil/extraction process/atmospheric distillation/analyse of GC-MS引用本文复制引用
基金项目
福建省属公益类科研院所基本科研专项(2021R1030003)
福建省属公益类科研院所基本科研专项(2023R1028001)
福建省属公益类科研院所基本科研专项(2020R1030004)
福建省农业科学院创新团队项目(CXTD2021001-2)
出版年
2024
国家科技期刊平台
NSTL
万方数据