Study on rapid determination method of iron content in animal-source feed materials
The study aimed to establish a method for the rapid detection of iron content in animal-source feed materials using spectrophotometry.Two spectrophotometric methods(the phenanthroline method and the potassium thiocyanate method)were used to detect iron in the ash of animal-source feed materials.The results showed that the test determined that the iron in the raw material exists in the form of Fe3+,and the potassium thiocyanate method was selected as the detection method for the subsequent test.The optimal conditions for the optimized potassium thiocyanate method were as follows:Ashing time of 3.5 hours,detection wavelength of 480 nm,volume of potassium thiocyanate solution 1.0 mL,color development time of 10 minutes,and color development temperature of 30 ℃,with no need to avoid light during the color development process.Under these parameters,the standard curve has a good linear relationship in the range of 1～20 mg/L(R2＞0.999 0),with the equation y=0.071 5x+0.009(R2=0.999 4).The potassium thiocyanate method had good stability,high accuracy,and strong resistance to interference,with a relative standard deviation of 1.03％～2.05％within batches,and the recovery rate of 98.02％～101.03％for six batches of chicken meal samples spiked with iron.Na+,K+,and Ca2+at concentrations 10 to 50 times that of Fe3+did not interfere with the detection of Fe3+content,making it feasible to replace atomic absorption spectrophotometry for rapid detection.The two methods were used to simultaneously determine the iron content in 11 types of animal-source feed materials samples,with no significant difference in results(β＞0.05).The study indicates that the potassium thiocyanate method established in the experiment can be used for the rapid detection of iron content in animal feed ingredients for pet food.
iron content determinationanimal-source feed materialsrapid determinationpotassium thiocyanate methodphenanthroline method
国家科技期刊平台
NSTL
万方数据
