Effects of balanced methionine and lysine on growth performance and nitrogen metabolism of Wanxi White geese under different protein levels
The experiment aims to investigate the effects of balanced methionine and lysine at different dietary protein levels on the growth performance and nitrogen metabolism of early Wanxi White geese.A total of 180 one-day-old Wanxi White geese were selected,with half males and half females,and randomly divided into six groups with five replicates in each group,with six geese in each replicate.The experimental period was 28 days.The experiment adopted a two factor crossover design,with feed protein levels of 20％,18％,and 16％,respectively,to balance lysine or methionine.The results showed that compared with the balanced lysine group,the balanced methionine group increased daily feed intake(ADFI)and daily weight gain(ADG)(P＜0.01),the ADFI and ADG levels in the 20％protein level group were higher than those in the other two groups(P＜0.01).The F/G of the 18％protein level group was extremely lower than the other two groups(P＜0.01).There was interaction between amino acid and protein levels in ADFI,ADG and F/G(P＜0.01).The intake of nitrogen,deposition of nitrogen,and nitrogen utilization efficiency in the balanced lysine group were extremely higher than those in the balanced methionine group(P＜0.01).The 20％protein level group had extremely higher nitrogen intake than the other two groups(P＜0.01),significantly higher nitrogen excretion than the other two groups(P＜0.05),and significantly lower nitrogen deposition and utilization efficiency than the other two groups(P＜0.01).There was an interaction between amino acids and protein levels of nitrogen intake and deposition(P＜0.01).The balanced methionine group significantly increased the utilization rates of methionine(P＜0.01),while the utilization rates of amino acids at the 20％protein level decreased(P＜0.01).There was an interaction between the utilization rates of amino acids at the protein and amino acid levels(P＜0.01).It was found that differential metabolites were mainly enriched in lipid,peptide,and organic acid pathways.The 18％protein level+balanced amino acid group enriched in pathways such as butyric acid metabolism,neuroactive ligand receptor interactions,ABC transporters,glycerophospholipid metabolism,and alpha linolenic acid metabolism.Lysine was the first limiting amino acid in 1～28 day old Wanxi White geese,and 18％protein level+balanced lysine(1.00％Lys+0.28％Met)was more conducive to the growth,development,and nitrogen metabolism of 1～28 day old Wanxi White geese.The study indicates that dietary lysine can upregulate characteristic metabolites significantly positively correlated with nitrogen metabolism(such as butyrate,deoxyadenosine,guanine,betaine,etc.),while methionine can upregulate characteristic metabolites significantly negatively correlated with nitrogen metabolism(such as histidine,creatine,3-hydroxybutyrate,xanthine,etc.).
lysinemethioninelow protein dietnitrogen metabolismblood metabolomicsWanxi White goose
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