Effect of dietary supplementation with Lactobacillus brucei and Bacillus subtilis on reproductive performance and ovarian development of Guangxi Ma chickens
The experiment aimed to study the effects of adding Lactobacillus brucei and Bacillus subtilis live bacterial liquid in the diet on the reproductive performance and ovarian development of Guangxi Ma chickens during the peak egg-laying period.A total of 480 healthy Guangxi Ma chickens at 27 weeks of age during the peak egg-laying period were randomly divided into four groups,with three replicates per group,each replicate consisting of 40 chickens.The trial lasted for nine weeks.The control group was fed a basic diet,the M1 group was supplemented with a live bacterial liquid of Lactobacillus brucei and Bacillus subtilis in a 1∶1 ratio in the basic diet,the L1 group was supplemented with Lactobacillus brucei live bacterial liquid in the basic diet,and the B1 group was supplemented with Bacillus subtilis live bacterial liquid in the basic diet,with the viable bacteria count of the probiotic preparation being 4.5×108 CFU/mL.The results showed that compared with the control group,the egg-laying rate of the M1 group significantly increased(P＜0.05)from the 2nd to the 8th week,and the average egg-laying rate and the rate of qualified breeding eggs significantly increased(P＜0.05).The levels of reproductive hormones such as serum E,PROG,FSH,and LH in the M1 group,as well as the relative expression levels of ovarian ER mRNA,PR mRNA,FSHR mRNA,and LHR mRNA,were all significantly increased(P＜0.05).The study indicates that the addition of a mixed bacterial liquid of Lactobacillus brucei and Bacillus subtilis in the diet can improve the reproductive performance of Guangxi Ma breeder chickens during the peak egg-laying period and promote the maturation of follicles and the development of the ovaries.
Lactobacillus buchneriBacillus subtilisGuangxi Ma chickensreproductive performanceovarian development
国家科技期刊平台
NSTL
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