Study on extraction of total saponins and polysaccharides in Shenmei Wuwei powder and in vitro activities
The experiment was to explore the optimal process conditions and in vitro activity for the joint extraction of total saponins and polysaccharides from Shenmei Wuwei powder.The extraction mass fraction of total saponins and polysaccharides of Shenmei Wuwei powder was used as the investigation index by optimizing the extraction solvent concentration and soaking time,the optimal extraction process was determined,and the antioxidant and antitumor activities of Shenmei Wuwei Powder were determined by determining the scavenging rate of hydroxyl radicals and the inhibition rate of nitrite in total saponin extract and polysaccharide extract.The results showed that under optimal parameter conditions,the extraction rates of total saponins in the four chromatographic columns were 92.65％,98.99％,102.68％,and 104.31％,the paste yield was 10.00％,and the total saponin content was 34.99 mg/g.The extraction rate of polysaccharides was 93.57％,98.20％,100.77％and 101.27％,the ointment yield was 12.55％,and the polysaccharide content in dry extract was 19.20 mg/g.The IC50 values of saponin extract and polysaccharide extract for scavenging hydroxyl radicals were higher than those of control vitamin C,indicating that they had antioxidant activity in vitro and certain nitrite inhibition.The study indicates that the total saponins and polysaccharides are extracted simultaneously by circulating column chromatography from the Shenmei Wuwei powder is high,and the operation is simple and easy,which provides reference value for the development of feed additives in animal husbandry.
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