Effects of Yimu Shenghuasan on serum hormones and TLR4/NF-κB signaling pathway related factors in LPS-induced endometritis in mice
The experiment is designed to explore the impact of Yimu Shenghuasan on the serum hormone levels and the expression levels of factors related to the TLR4/NF-κB signaling pathway in a mouse model of endometritis.A total of 40 female mice,with similar age,body size,and weight,were randomly divided into four groups with 10 replicates per group and one mouse per replicate.Mice in the control group were instillated with 20 μL normal saline,the model group was instillated with 25 μL lipopolysaccharide(LPS,1 g/L),the drug group was instillated with 2.0 g/kg Yimu Shenghuasan,and the treatment group was instillated with 25 μL LPS+2.0 g/kg Yimu Shenghuasan,continuously gavage for three days,twice a day.The contentrations of estradiol(E2),progesterone(Pg),luteinizing hormone(LH),and follicle-stimulating hormone(FSH)in the serum was detected by radioimmunoassay,the contentrations of interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-8(IL-8),tumor necrosis factor-α(TNF-α)in the serum was detected by ELISA method,and the expression of factors related to the TLR4/NF-κB signaling pathway,including Toll-like receptor 4(TLR4),myeloid differentiation factor(MyD88),nuclear factor-KB p65(NF-κB p65),IL-1β,IL-6,IL-8,TNF-α mRNA was detected by RT-PCR method.The results showed that compared with the control group,the serum E2,LH,and FSH contentrations in the model group was significantly reduced,while Pg content increased,the secretion and mRNA gene expression of IL-1β,IL-6,IL-8,and TNF-α were significantly increased,and the expression of TLR4,MyD88,NF-κB p65 mRNA was significantly upregulated.Compared with the model group,the serum E2,LH,and FSH content in the treatment group was significantly increased,Pg content reduced,the secretion and mRNA gene expression of IL-6,IL-8,IL-1β,and TNF-αwere significantly reduced,and the expression of TLR4,MyD88,NF-κB p65 mRNA was significantly downregulated.The study indicates that Yimu Shenghuasan,by regulating the content of hormones and inhibiting the changes in the levels of factors related to the TLR4/NF-κB signaling pathway,has a relieving effect on LPS-induced mouse endometriosis.
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