枯草芽孢杆菌BYA-KC-4产表面活性素发酵条件的优化
Optimizing fermentation conditions for production of surfactin from Bacillus subtilis BYA-KC-4
李高强 1张旭斌 1牛俊轲 1刘永立 1张锋 1高原源 1李梦洁 1贵文娟1
作者信息
- 1. 焦作市佰役安生物工程有限公司,河南焦作 454350;河南省饲用替抗工程技术研究中心,河南焦作 454350
- 折叠
摘要
试验旨在对高产表面活性素菌株枯草芽孢杆菌BYA-KC-4的发酵条件进行优化,筛选出最佳的生产表面活性素的条件.试验通过单因素试验确定影响产表面活性素的因素水平.采用Box-Behnken中心组合设计,进一步优化了发酵条件参数.结果表明,在最佳发酵条件下,即33 ℃、初始pH值6.8、接种量4.8％、转速201 r/min,表面活性素的产量达到了 560.25 mg/L,相比优化前的425.36 mg/L,提高了 1.32倍.研究表明,试验可为枯草芽孢杆菌BYA-KC-4产表面活性素工业化生产提供参考.
Abstract
The experiment aimed to optimize the fermentation conditions for the high-yielding surfactin-producing strain Bacillus subtilis BYA-KC-4 and to screen for the best conditions for surfactin production.The experiment determined the levels of factors affecting surfactin production through single-factor tests.The Box-Behnken central composite design was used to further optimize the fermentation condition parameters.The results showed that under the optimal fermentation conditions,namely a temperature of 33℃,an initial pH value of 6.8,an inoculum volume of 4.8％,and a rotation speed of 201 r/min,the yield of surfactin reached 560.25 mg/L.Compared with the 425.36 mg/L before optimization,it increased by 1.32 times.The study indicates that the experiment can provide a reference for surfactin production by Bacillus subtilis BYA-KC-4 and for industrial production.
关键词
表面活性素/枯草芽孢杆菌/响应面优化/发酵条件Key words
surfactin/Bacillus subtilis/response surface optimization/fermentation conditions引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据