Optimization of flavonoid extraction process from chicory leaves and analysis of its antioxidant activity by response surface methodology
The study was to enhance the feed value of chicory leaves.Through response surface methodology,the experiment optimized the extraction process of flavonoids from chicory leaves and evaluates their antioxidant activity.Based on single-factor experiments,the design and optimization of the Box-Behnken design were conducted with the response variable being the yield of flavonoids,to analyze the effects of the liquid-to-solid ratio,extraction temperature,and extraction time on the yield of flavonoids in chicory leaves.The results showed that the optimized conditions for the extraction of chicory leaf flavonoids were a liquid-to-solid ratio of 37.0 mL/g,an extraction time of 59.2 minutes,and an extraction temperature of 43 ℃.Under these conditions,the yield of chicory leaf flavonoids was 3.09％,which was basically consistent with the predicted value,indicating that this optimization method was reasonable and reliable.In vitro antioxidant activity tests showed that the scavenging ability of chicory leaf flavonoids on DPPH and ABTS+radicals was concentration-dependent.The study suggests that chicory leaf flavonoids have strong in vitro antioxidant capacity and potential for feed additive development.
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