Effect of different material to water ratios on quality of mixed-culture fermentation of corn husk
The study aims to explore the impact of different material to water ratios on the quality of corn husk during mixed microbial fermentation.The experiment used a solid-state fermentation process,setting up four groups with material to water ratios of 30%(Group Ⅰ),40%(Group Ⅱ),50%(Group Ⅲ),and 60%(Group Ⅳ),with four replicates for each group.Corn husk,rice bran,and soybean meal were mixed evenly in a ratio of 20∶3∶3,then mixed with a microbial solution containing Bacillus coagulans,Lactobacillus crispatus,and Bacillus subtilis(1∶1∶1)at a seeding rate of 7%,and fermented for five days to investigate the effect of different material to water ratios on the fermentation effect of feed.The results showed that under the condition of a 50%material to water ratio,the total viable count was 3.5 × 108 CFU/g,which is 58 times that before fermentation.The contents of vomitoxin and dry matter(DM)in Group Ⅲwere significantly lower than those in Group Ⅰ,Group Ⅱ and Group Ⅳ(P<0.05),the content of neutral detergent fiber(NDF)in Group Ⅲ was significantly lower than that in Group Ⅰ and Group Ⅱ(P<0.05),and the pH value of Group m was 4.18.The study indicates that the optimal material to water ratio for solid-state fermentation of corn husk by mixed microbial cultures is 50%.Under this condition,the fermented feed has a uniform and loose texture with a rich aroma.
material to water ratiofermented corn huskmixed probioticsquality assessment