Effects of compound probiotics on growth performance,immunity and intestinal barrier related gene expression in piglets
The experiment aimed to study the effects of different levels of compound probiotics on the growth performance,immunity,and intestinal barrier function of piglets.A total of 160'Duroc×Landrace×Yorkshire'weaned piglets at 21 days of age were randomly divided into four groups,with four replicates in each group,and 10 piglet in each replicate.The control group,test group 1,test group 2,and test group 3 were fed diets containing 0,0.1％,0.2％,and 0.4％compound probiotics,respectively.The preliminary trial lasted for one week,and the formal experiment lasted for six weeks.The results showed that compared with the control group,the final weight and average daily weight gain of piglets in the test group 2 and group 3 increased significantly by 13.64％,11.81％and 20.43％,16.22％(P＜0.05),respectively,and the average daily feed intake increased by 6.98％,7.71％(P＜0.05).The feed to gain ratio of all the test groups decreased by 7.54％,11.06％,and 7.54％(P＜0.05),and the diarrhea rate decreased by 29.04％,44.03％,and 49.53％(P＜0.05).Compared with the control group,the levels of IgA and IgG in the serum of piglets in the test group 2 and group 3 increased significantly(P＜0.05).The level of IL-6 decreased significantly(P＜0.05),the level of IL-10 in the serum of all the test groups increased significantly(P＜0.05),and the level of TNF-α in the serum of piglets in the test group 2 decreased significantly(P＜0.05).Compared with the control group,the level of D-LA in the test group 2 decreased significantly(P＜0.05),and the levels of ET in the test group 2 and group 3 decreased significantly(P＜0.05).The expression of Occludin in the ileum of the test group 2 and group 3 increased significantly(P＜0.05),the expression of ZO-1 and TRAF6 in the test group 2 increased significantly(P＜0.05),the expression of Occludin,TLR2,and NF-κB in the colon of the test group 2 and group 3 increased significantly(P＜0.05),and the expression of TLR4 in the test group 2 increased significantly(P＜0.05).The study indicates that the addition of an appropriate amount of compound probiotics to the diet can improve the growth performance and immunity of piglets and enhance the function of the intestinal mucosal barrier.The suitable addition level of compound probiotics is 0.2％.
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