Isolation and identification of lactic acid bacteria from Cyperus esculentus L.stem and leaf silage
The experiment was to isolate and identify the dominant lactic acid bacteria(LAB)from the silage of Cyperus esculentus L.,so as to provide favourable LAB resources for improving the silage quality of Cyperus esculentus L.Eight LAB strains were finally isolated and identified by microbial culture and 16S rDNA sequence analysis.The eight isolated LAB strains were used for the silage test of Cyperus esculentus L,and the nutritional components were measured at 3,7,15,30,60 days of the silage process.The results showed that H1 and H7 were Lactobacillus plantarum,M7,M13 and M15 were Pediococcus acidilactici,M1 and M11 were Enterococcus faecalis,and R12 was Weissella paramesenteroides.Except for Pediococcus acidilactici,which could not grow at pH value of 3,the other three strains all exhibited strong acid and salt tolerance,had a broad temperature adaptation range,and feature a variety of fermentable carbon sources.The silage of Cyperus esculentus L.made with the eight isolated strains of LAB had a high crude protein content,and low levels of neutral detergent fiber and acid detergent fiber,which had good nutritional quality.The study indicates that the study can provide strain resources for the preparation of Cyperus esculentus L.silage additives.
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