Evaluation of alkali stress of alfalfa varieties at germination period
In order to screen high quality alfalfa varieties suitable for planting in northeast China,130 alfalfa varieties at home and abroad and newly selected varieties were used as the research materials.The seeds at germination stage were alkali stress treated with 30 mmol/L Na2CO3 and 50 mmol/L NaHCO3,respectively.Comprehensive evaluation of alkali stress of alfalfa varieties were analyzed by detecting germination index and subordinate function analysis.The results showed that the average relative germination rates of 130 alfalfa species under Na2CO3 and NaHCO3 stress were 68.54%and 79.17%,respectively.The relative germination vigor were 57.30%and 65.78%respectively.The relative root length was 23.70%and 17.61%,respectively.The relative bud length was 53.21%and 46.37%,respectively.Through the analysis of membership function,it was found that the seeds of seven varieties of LJ112,LJ66,LJ33,5010,DARA,Nongjing 1 and Juneng salt-tolerant had good germination trend under two alkali stresses.By calculating the comprehensive membership function values of 130 alfalfa varieties under two alkali stresses,it can be seen that Nongjing 1 and LJ33 have the best germination and growth under two alkali stresses.The study indicates that this experiment is beneficial for guiding the cultivation of alfalfa and providing germplasm resources for the breeding of alkali tolerance alfalfa varieties.