Effects of different forage on intestinal microorganisms of Kazakh horse
The aim of this experiment was to study the difference of intestinal microflora of Kazakh horses in grass group and hay group after transfer.Twenty Kazakh mares aged five to six years old under uniform feeding and management conditions in the Tacheng area of Xinjiang were selected and divided into two groups:Fresh Grass Group(QC group)and Dry Grass Group(GC group).They were fed 2 kg of concentrate feed per horse per day.QC group consumed fresh grass daily,while the GC group consumed dry grass daily.At 30 days of the experiment,fecal samples of all horses were collected,and the characteristic sequences of microbial species such as 16S amplified by PCR were detected by high-throughput sequencing technology.There were 28 865 OTU in fecal flora of Kazakh horses in the two groups,and the OTU amount in fecal flora of Kazakh horses in QC group was higher than that in GC group.The results showed that Firmicute,Bacteroidota,Proteobacteria,Verrucomicrobiota,and Spirochaetota were the dominant bacteria in the manure of Kazakh horses in the two groups.The dominant bacteria genera were Solibacillus,Acinetobacter,Rikenellaceae_RC9_gut_group,Treponema,and Ruminococcaceae NK4A214_group.Compared with the GC group,the relative abundances of Actinobacteriota and Rummeliibacillus in feces of Kazakh horses in QC group were significantly increased(P＜0.05).LEfSe analysis showed that 65 kinds of bacteria were significantly different in fecal flora of Kazakh horses in the two groups,among which 36 kinds of bacteria were significantly up-regulated in QC group and 29 kinds of bacteria were significantly up-regulated in GC group.The study indicates that the composition structure and community of fecal flora of Kazakh horses were affected by different forage,but the diversity and richness of intestinal flora did not change significantly.More important microbial groups played an important role in fecal flora of Kazakh horses in QC group than in GC group.
国家科技期刊平台
NSTL
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