Research on process of producing protein feed by solid-state fermentation of feather meal with microbial enzyme synergy
The study explored the feasibility of producing protein feed by microbial enzyme synergy fermentation of feather meal and to determine the process parameters for this production.The keratin-degrading bacterial strains selected from the laboratory were combined with commercially available keratinase enzyme preparations to utilize microbial enzyme synergy in solid-state fermentation of feather meal.The in vitro pepsin digestibility was used as the index to analyze the fermentation time,inoculation amount,microbial enzyme ratio,and water addition through single-factor and orthogonal test analysis.The results showed that the optimal fermentation process was obtained:The water addition to feather meal was 45％,the microbial enzyme ratio was 1∶1,the inoculation amount was 6％,and the fermentation time was 7 days.After fermentation,the in vitro pepsin digestibility of feather meal was 63.97％,which was significantly improved by 15.22％compared to before fermentation(P＜0.01),and the soluble protein content was 3.51 g/L,which was significantly increased by 80％compared to before fermentation(P＜0.05).The study indicates that under the optimal process parameters,using microbial enzyme synergy in solid-state fermentation of feather meal can efficiently produce protein feed and enhance the feed value of feather meal.
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