The study explored the feasibility of producing protein feed by microbial enzyme synergy fermentation of feather meal and to determine the process parameters for this production.The keratin-degrading bacterial strains selected from the laboratory were combined with commercially available keratinase enzyme preparations to utilize microbial enzyme synergy in solid-state fermentation of feather meal.The in vitro pepsin digestibility was used as the index to analyze the fermentation time,inoculation amount,microbial enzyme ratio,and water addition through single-factor and orthogonal test analysis.The results showed that the optimal fermentation process was obtained:The water addition to feather meal was 45％,the microbial enzyme ratio was 1∶1,the inoculation amount was 6％,and the fermentation time was 7 days.After fermentation,the in vitro pepsin digestibility of feather meal was 63.97％,which was significantly improved by 15.22％compared to before fermentation(P＜0.01),and the soluble protein content was 3.51 g/L,which was significantly increased by 80％compared to before fermentation(P＜0.05).The study indicates that under the optimal process parameters,using microbial enzyme synergy in solid-state fermentation of feather meal can efficiently produce protein feed and enhance the feed value of feather meal.
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