菌酶协同固态发酵羽毛粉生产蛋白饲料的工艺研究
Research on process of producing protein feed by solid-state fermentation of feather meal with microbial enzyme synergy
贾小平 1董宝花 2龚建刚 3刘芳宇 1何俊鑫 1王琪 1冯志华 1张海华4
作者信息
- 1. 河北农业大学动物科技学院,河北保定 071000
- 2. 南京农业大学动物科技学院,江苏南京 210095
- 3. 河北农业大学食品科技学院,河北保定 071000
- 4. 河北科技师范学院,河北秦皇岛 066000
- 折叠
摘要
研究旨在探讨菌酶协同发酵羽毛粉生产蛋白饲料的可行性,并确定生产工艺参数.将实验室筛选得到的角蛋白高效降解菌株与市售角蛋白酶结合,协同固态发酵羽毛粉.以体外胃蛋白酶消化率为考察指标,对发酵时间、接种量、菌酶比例、加水量进行单因素和正交试验分析.结果显示,最佳发酵工艺为羽毛粉加水量45％、菌酶比例1∶1、接种量6％、发酵时间7 d.发酵后羽毛粉的体外胃蛋白酶消化率为63.97％,较发酵前提高了15.22％(P＜0.01);可溶性蛋白含量为3.51 g/L,较发酵前提高了80％(P＜0.05).研究表明,在最佳工艺参数下,利用菌酶协同固态发酵羽毛粉可以高效生产蛋白饲料,提高了羽毛粉的饲用价值.
Abstract
The study explored the feasibility of producing protein feed by microbial enzyme synergy fermentation of feather meal and to determine the process parameters for this production.The keratin-degrading bacterial strains selected from the laboratory were combined with commercially available keratinase enzyme preparations to utilize microbial enzyme synergy in solid-state fermentation of feather meal.The in vitro pepsin digestibility was used as the index to analyze the fermentation time,inoculation amount,microbial enzyme ratio,and water addition through single-factor and orthogonal test analysis.The results showed that the optimal fermentation process was obtained:The water addition to feather meal was 45％,the microbial enzyme ratio was 1∶1,the inoculation amount was 6％,and the fermentation time was 7 days.After fermentation,the in vitro pepsin digestibility of feather meal was 63.97％,which was significantly improved by 15.22％compared to before fermentation(P＜0.01),and the soluble protein content was 3.51 g/L,which was significantly increased by 80％compared to before fermentation(P＜0.05).The study indicates that under the optimal process parameters,using microbial enzyme synergy in solid-state fermentation of feather meal can efficiently produce protein feed and enhance the feed value of feather meal.
关键词
羽毛粉/菌酶协同/固态发酵/蛋白饲料Key words
feather meal/microbial enzyme synergy/solid-state fermentation/protein feed引用本文复制引用
基金项目
河北农业大学2022-2023大学生创新创业训练计划项目(2022047)
河北省现代农业产业技术体系蛋鸡肉鸡(家禽)营养与饲料(HBCT2023210202)
河北省重点研发计划项目乡村振兴技术创新专项(22327308D)
出版年
2024
国家科技期刊平台
NSTL
万方数据