扶芳藤黄酮提取工艺优化及其抗疲劳活性研究
Study on optimization of extraction process and anti-fatigue activity of flavonoids from Euonymus fortunei(Turcz.)Hand.-Mazz.
张苑 1程玥 1谢宗固 2司红彬2
作者信息
- 1. 广西大学动物科学技术学院广西畜禽繁育与疾病防控重点实验室,广西南宁 530004
- 2. 广西大学动物科学技术学院广西畜禽繁育与疾病防控重点实验室,广西南宁 530004;广西壮族自治区牧草工作站,广西南宁 530004
- 折叠
摘要
试验旨在优化扶芳藤黄酮提取工艺,研究其抗疲劳和抗氧化作用.试验以扶芳藤黄酮提取率为指标,对液料比、乙醇浓度、提取时间、超声功率等因素进行了单因素试验,在此基础上进行了响应面优化设计,考察了扶芳藤的抗疲劳效果.将50只小鼠随机分为空白对照组、阳性对照组(复方扶芳藤合剂)、疲劳模型组以及扶芳藤黄酮提取物低、高(200 mg/kg和400mg/kg)剂量组,每组10只,进行小鼠抗疲劳试验.结果显示,扶芳藤黄酮最佳提取条件为液料比55mL/g、乙醇浓度60％、提取时间73 min、超声功率175 W,在此条件下黄酮得率为5.94％,与预测值基本一致.扶芳藤黄酮能够有效延长小鼠负重游泳时间,降低血清中乳酸(LD)和尿素氮(BUN)含量,增加小鼠体内肝糖原和肌糖原水平,增强超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性,降低丙二醛(MDA)含量.研究表明,扶芳藤黄酮的抗疲劳和抗氧化效果较好,可作为缓解动物疲劳和氧化应激的饲料添加剂.
Abstract
The experiment aimed to optimize the extraction process of Euonymus fortunei(Turcz.)Hand.-Mazz.flavonoids and study their anti-fatigue and antioxidant effects.The experiment took the extraction rate of Euonymus fortunei(Turcz.)Hand.-Mazz.flavonoids as the index and conducted single-factor experiments on factors such as liquid-material ratio,ethanol concentration,extraction time,and ultrasonic power.Based on this,a response surface optimization design was carried out to investigate the anti-fatigue effect of Euonymus fortunei(Turcz.)Hand.-Mazz.Fifty mice were randomly divided into a blank control group,a positive control group(Euonymus fortunei(Turcz.)Hand.-Mazz.compound mixture),a fatigue model group,and low and high dose groups of Euonymus fortunei(Turcz.)Hand.-Mazz.flavonoid extract(200 mg/kg and 400 mg/kg),with 10 mice in each group,for the anti-fatigue experiment in mice.The results showed that the optimal extraction conditions for Euonymus fortunei(Turcz.)Hand.-Mazz.flavonoids had a liquid-material ratio of 55 mL/g,an ethanol concentration of 60％,an extraction time of 73 min,and an ultrasonic power of 175 W,under which the flavonoid yield was 5.94％,which was basically consistent with the predicted value.Euonymus fortunei(Turcz.)Hand.-Mazz.flavonoids could effectively extend the swimming time of mice carrying weights,reduce the concentrations of lactic acid(LD)and blood urea nitrogen(BUN)in serum,increase the levels of liver glycogen and muscle glycogen in mice,enhance the activities of superoxide dismutase(SOD),catalase(CAT),glutathione peroxidase(GSH-Px),and reduce the content of malondialdehyde(MDA).The study shows that Euonymus fortunei(Turcz.)Hand.-Mazz.flavonoids have good anti-fatigue and antioxidant effects and can be used as a feed additive to alleviate animal fatigue and oxidative stress.
关键词
扶芳藤/黄酮/抗疲劳/抗氧化Key words
Euonymus fortunei(Turcz.)Hand.-Mazz./flavonoids/anti-fatigue/antioxidation引用本文复制引用
基金项目
中央引导地方专项(桂科计字202355号)
国家自然科学基金联合基金(U22A20523)
福喜乐科技专项(20210003)
广西大学自科创新倍增计划(2024BZPT020)
出版年
2024
国家科技期刊平台
NSTL
万方数据