Influence of three sugars from Alhagi honey on immunomodulation and intestinal morphology structure in mice
The experiment aimed to explore the effects of three main sugars in Alhagi honey on the immune regulation and jejunal morphological in mice.Alhagi honey polysaccharides(AHP),water-soluble small molecular sugars(AHWS),and alcohol-soluble small molecular sugars(AHAS)were obtained by water extraction,alcohol precipitation,and dialysis methods,respectively.A total of 40 Kunming mice were selected and randomly divided into four groups,namely the control group(NC group)and the experimental groups(AHAS group,AHWS group,AHP group),with ten replicates per group and one mouse per replicate.The NC group was gavage-fed with physiological saline,while the experimental groups were gavage-fed with 400 mg/(kg·d)of AHAS,AHWS,and AHP 0.4 mL/mice,once a day for 14 consecutive days.The results showed that the total sugar yields of AHAS,AHWS,and AHP were 42.40％,8.20％,and 1.90％,respectively.Compared with NC group,the thymus weight in experimental groups was significantly increased(P＜0.05),the spleen weight and spleen index in AHAS group and AHP group were significantly increased(P＜0.05),and the thymus index in AHAS group and AHWS group were significantly increased(P＜0.05).Compared with NC group,the serum immunoglobulin G(IgG),interleukin-4(IL-4),interleukin-17(IL-17)and tumor necrosis factor-α(TNF-α)concentrations in experimental groups were significantly increased(P＜0.05).The villus length and crypto-wool ratio of jejunum in AHAS and AHP groups were significantly increased(P＜0.05),while the crypt depth was significantly decreased(P＜0.05).The number of jejunal intestinal epithelial lymphocytes(IELs)was significantly increased in all experimental groups(P＜0.05).The study indicates that AHAS,AHWS,and AHP in Alhagi honey have good safety,can enhance the cellular and humoral immunity of the body,and improve the intestinal morphology of mice.
Alhagi honeywater-soluble small molecule sugarsalcohol-soluble small molecule sugarsimmunomodulationmice
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