Effects of replacement of dietary fish oil by linseed oil on growth performance and muscle metabolic capacity of large yellow croaker(Larimichthys crocea)
The purpose of this study was to investigate the impacts of replacement of dietary fish oil by linseed oil on the growth performance,feed utilization,and muscle metabolic capacity of large yellow croaker(Larimichthys crocea).A total of 360 juvenile large yellow croaker with an initial weight of(3.89±0.01)g were randomly divided into two groups,with three replicates per group and 60 fish per replicate.Two isonitrogenous and isolipidic diets were formulated,namely the fish oil group(FO group,with a fish oil addition of 6％)and the linseed oil group(LO group,where linseed oil completely replaced fish oil).The experimental period was 10 weeks.The results showed that the final weight and weight gain rate of the large yellow croaker in the LO group were significantly lower than those in the FO group(P＜0.05),and the feed conversion ratio was significantly higher than that in the FO group(P＜0.05).Compared with the FO group,the concentrations of linoleic acid and α-linolenic acid in the muscle of the LO group were significantly increased(P＜0.05),while the concentrations of eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)were significantly decreased(P＜0.05).The substitution of fish oil with linseed oil significantly affected the expression of genes related to AMPK/SIRT1/PGC-1α pathway,and glucose and lipid metabolism in muscle(P＜0.05).The activity of lactate dehydrogenase and the concentrations of glucose-6-phosphate and lactate in the muscle of the LO group were significantly higher than those in the FO group(P＜0.05),while the activities of citrate synthase and cytochrome c oxidase were significantly lower than that in the FO group(P＜0.05).The study indicates that the total replacement of dietary fish oil with linseedoil can inhibit growth performance and feed utilization,reduce the content of muscle EPA and DHA and induced the declines in the AMPK/SIRT1/PGC-1α pathway-related genes expression andmitochondrial aerobic metabolism in muscle of large yellow croaker.
万方数据
维普
