Effects of feeding frequency on growth performance and gut microbiota of large-mouth bass fry after acclimation with compound feed
The study was to explore the effects of daily feeding frequency on the growth performance,feed utilization,digestive enzyme activity,and gut microbiota of large-mouth bass fry after domestication and feeding with a combination feed.The experiment selected 4 500 large-mouth bass with an initial weight of(0.65±0.01)g,randomly divided into five feeding frequency groups(1 to 5 meals/day),named F1 group,F2 group,F3 group,F4 group,and F5 group,with three replicates per group and 300 fish per replicate.The trial period was 30 days.The results showed that the final weight,specific growth rate,daily feeding rate,and condition factor of the fish in F1 group were significantly lower than those in the other groups(P＜0.05),while the survival rates in F3 group and F4 group were significantly increased(P＜0.05).The moisture content in the fish bodies of F1 group was increased(P＜0.05),and the crude fat content was lower than that in the other groups(P＜0.05).The intestinal protease activity in F1 group was significantly higher than in the other groups(P＜0.05).The relative abundance of Aeromonas and Acinetobacter in the intestine of F1 group was significantly increased(P＜0.05),while the relative abundance of Cetobacterium was significantly lower than in the other groups(P＜0.05).The study indicates that based on growth performance and survival rate,the appropriate daily feeding frequency for large-mouth bass fry fed with compound feed is 3～4 meals per day.
万方数据
维普
