Bioinformatics,gene expression,and activity analysis of wheat amylase
The purpose of this experiment was to explore the structural characteristics,expression and enzymatic activity changes during germination of wheat α-amylase and β-amylase.The structural characteristics of α-amylase andβ-amylase in wheat were predicted by bioinformatics,fluorescence quantitative PCR was used to detect the expression level of α-amylase and β-amylase gene in wheat seeds during the germination process,and their enzymatic activity was measured.The results showed that wheat α-amylase was an alkaline protein,β-amylase was an acidic protein,α-amylase had a signal peptide and a transmembrane region,while β-amylase did not,and the secondary and tertiary structures and number of phosphorylation sites of both amylases were similar.When wheat sprouted,the expression level of α-amylase gene first increased and then decreased,while the expression level of β-amylase gene was at a low level,the expression ofα-amylase gene was induced by low concentration of salt alkali and inhibited by high concentration of salt alkali,the expression level of β-amylase gene increased with increasing salt alkali concentration,the activity of α-amylase andβ-amylase significantly increased in the late stage and early stage of germination,respectively.The results indicated that wheat α-amylase was a secreted membrane protein,β-amylase was a stroma free protein,the stress responses of α-amylase and β-amylase to salt alkali were different,and they played a major role in the late and early stages of wheat germination respectively.
万方数据
维普
