中药复方多糖对肉鸡生长性能、免疫调节及肠道菌群的影响
Effects of traditional Chinese medicine compound polysaccharides on growth performance,immune regulation,and intestinal flora of broilers
刘思莹 1杨侃侃 2苏顺达 1李逸飞 1毕艺萌 1叶力维 1李敬双 1于洋1
作者信息
- 1. 锦州医科大学,辽宁锦州 121001
- 2. 辽宁省农业经济学校,辽宁锦州 121001
- 折叠
摘要
试验旨在探究中药复方多糖(TCMCP)对肉鸡生长性能、免疫调节及肠道菌群的影响.选取900只1日龄肉仔鸡,设空白对照组(BCG)饲喂基础饲粮、阳性对照组(PCG)饲喂基础饲粮并添加10 mg/kg盐酸左旋咪唑、不同浓度TCMCP组(0.5％、1.0％、2.0％TCMCPG组)饲喂基础饲粮并添加0.5％、1.0％和2.0％TCMCP.每组设3个重复,每个重复60只,试验期42 d.结果显示,与BCG组相比,0.5％、1.0％和2.0％TCMCPG组均极显著提高末重(P＜0.01);1.0％TCMCPG组极显著提高平均日增重(P＜0.01),0.5％和2.0％TCMCPG组均显著提高平均日增重(P＜0.05);0.5％、1.0％和2.0％TCMCPG组均极显著降低料重比(P＜0.01).与BCG组相比,1.0％和2.0％TCMCPG组均极显著提高血清中免疫球蛋白G(IgG)含量(P＜0.01),0.5％TCMCPG组显著提高血清中IgG含量(P＜0.05);1.0％TCMCPG组极显著提高血清中免疫球蛋白A(IgA)含量(P＜0.01);1.0％和2.0％TCMCPG组均显著提高血清中免疫球蛋白M(IgM)含量(P＜0.05).0.5％、1.0％和2.0％TCMCPG组均极显著提高胸腺指数、法氏囊指数和脾脏指数(P＜0.01),极显著提高血清中白细胞介素-2(IL-2)、白细胞介素-4(IL-4)和干扰素-γ(IFN-γ)含量(P＜0.01);使脾组织结构中淋巴细胞数量增多,体积增大.在肠道菌群方面,0.5％、1.0％和2.0％TCMCPG组均极显著降低空肠和盲肠中大肠杆菌数量(P＜0.01),极显著提高盲肠中乳酸菌数量(P＜0.01);1.0％TCMCPG组极显著提高空肠中乳酸菌数量(P＜0.01).研究表明,TCMCP能够提高肉仔鸡的免疫功能,改善肠道菌群结构,提高生长性能,1.0％TCMCPG效果最佳.
Abstract
The experiment aimed to study the impact of traditional Chinese medicine compound polysaccharides on the growth performance,immune moderation,and intestinal flora of broilers.A total of 900 1-day-old broilers were selected.The broilers in the blank control group(BCG)were fed a basal diet,and those in the positive control group(PCG)were fed a basal diet supplemented with 10 mg/kg levamisole hydrochloride,and those in different concentrations of TCMCP groups(0.5％,1.0％,and 2.0％TCMCPG)were fed a basal diet supplemented with 0.5％,1.0％,and 2.0％TCMCP.There were 3 replicates in each group and 60 broilers in each replicate.The experiment lasted for 42 days.The results showed that the final weight of 0.5％,1.0％,and 2.0％TCMCPG groups was extremely higher than the BCG group(P＜0.01).1.0％TCMCPG group extremely increased average daily gain(P＜0.01),0.5％and 2.0％TCMCPG groups significantly increased average daily gain(P＜0.05).The ratio of feed to gain in 0.5％,1.0％and 2.0％TCMCPG groups was extremely decreased(P＜0.01).Serum immunoglobulin G(IgG)content in 1.0％and 2.0％TCMCPG groups was extremely higher than the BCG group(P＜0.0l),and serum IgG content in 0.5％TCMCPG group was significantly increased(P＜0.05).1.0％TCMCPG group extremely increased the content of immunoglobulin A(IgA)(P＜0.01).Serum immunoglobulin M(IgM)content in 1.0％and 2.0％TCMCPG groups was significantly increased(P＜0.05).In 0.5％,1.0％and 2.0％TCMCPG groups,thymus,bursa,and spleen indexes were extremely increased(P＜0.01),and serum concentrations of interleukin-2(IL-2),interleukin-4(IL-4),and interferon-y(IFN-γ)were extremely increased(P＜0.01).The number and volume of lymphocytes in the splenic tissue structure increased.In terms of intestinal flora,0.5％,1.0％and 2.0％TCMCPG groups extremely decreased the number of Escherichia coli in jejunum and cecum(P＜0.01),and extremely increased the number of Lactobacillus in cecum(P＜0.01).The Lactobacillus number in jejunum was extremely increased in 1.0％TCMCPG group(P＜0.0l).The study indicates that TCMCP can improve the immune function,improve the enteric microbiome,and improve growth performance,1.0％TCMCPG has the best effect.
关键词
中药复方多糖/肉仔鸡/生长性能/免疫功能/肠道菌群Key words
traditional Chinese medicine compound polysaccharide/broilers/growth performance/immune function/intestinal flora引用本文复制引用
基金项目
2023年大学生创新创业计划训练项目(S202310160010)
2022年度辽宁省教育厅重点攻关项目(LJKZZ20220094)
出版年
2024
国家科技期刊平台
NSTL
万方数据