Effects of adding different levels of mulberry leaf polysaccharides to feed on growth performance,immunity,and digestive enzyme activities of Micropterus salmoides
The aim of the experiment was to investigate the effects of different levels of mulberry leaf polysaccharides on the growth performance,immunity,and digestive enzyme activities of Micropterus salmoides.Seven hundred and twenty healthy Micropterus salmoides weighing(25.05±1.06)g were selected for the experiment and randomly divided into four groups with three replicates per group and 60 Micropterus salmoides per replicate.The control group,0.5%MLP,1.0%MLP,and 2.0%MLP groups were fed experimental diets supplemented with 0,0.5%,1.0%,and 2.0%mulberry leaf polysaccharides,respectively.The experimental period lasted for eight weeks.The results showed that the final body weight,specific growth rate,and survival rate of Micropterus salmoides in the 1.0%MLP group were significantly higher than those in the control group(P<0.05),while the feed coefficient and viscera-somatic index were significantly lower than those in the control group(P<0.05).The complement C3 level in Micropterus salmoides in the 1.0%MLP group was significantly higher than that in the control group and 0.5%MLP group(P<0.05),the lysozyme activity in the 1.0%and 2.0%MLP groups was significantly higher than that in the control group and the 0.5%MLP group(P<0.05),and the activities of alkaline phosphatase and superoxide dismutase were significantly higher than those in the control group(P<0.05),the malondialdehyde content in the 1.0%and 2.0%MLP groups was significantly lower than that in the control group(P<0.05).The lipase,trypsin,and α-amylase activities in all experimental groups were significantly higher than those in the control group(P<0.05),with the highest digestive enzyme activity in the 1.0%MLP group.The study indicates that the addition of an appropriate amount of mulberry leaf polysaccharides to the feed can enhance the growth performance,non-specific immunity,and intestinal digestive enzyme activity of Micropterus salmoides,with the suitable addition level being 1.0%.