Study on optimization of extraction process and antioxidant activity of total flavonoids from dandelion
The aim of this study was to optimize the extraction process of total flavonoids from dandelion and study its antioxidant activity.The experiment investigates the effects of ethanol concentration,extraction temperature,and extraction time on the extraction yield of dandelion total flavonoids,and optimizes the extraction process of dandelion total flavonoids by orthogonal test.The results showed that the optimal extraction parameters were ethanol concentration of 60％,extraction temperature of 70℃ and extraction time of 45 min.Under these conditions,the extraction rate of total flavonoids from dandelion was 5.62％.The total flavonoids of dandelion had strong scavenging ability for DPPH radical and ABTS+radical,the IC50 values were 235.6 mg/L and 306.8 mg/L,respectively.The results show that it was feasible to optimize the ultrasonic extraction process by orthogonal test,total flavonoids of dandelion have strong antioxidant properties and are a high quality natural antioxidant substance.
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