Effects of compound combination of ginseng crude extract with schizophyllan or glucose oxidase on production performance and serum biochemical indexes of laying hens
The experiment aimed to investigate the effects of adding ginseng crude extract and its combination with schizophyllan or glucose oxidase on the egg production performance and serum biochemical indexes of laying hens.A total of 720 Roman Grey laying hens at 240 days of age were randomly divided into four groups,with six replicates per group and 30 hens per replicate.The control group was fed a basal diet,Group Ⅰ was fed a basal diet+0.2%ginseng crude extract,Group Ⅱ was fed a basal diet+0.2%ginseng crude extract+750 mg/kg schizophyllan,and Group Ⅲ was fed a basal diet+0.2%ginseng crude extract+100 g/t glucose oxidase.The trial lasted for 56 days.The results showed that on day 28 of the trial,the eggshell thickness of Group Ⅱ was significantly higher than that of the control group(P<0.05),and the substandard egg rate of Group Ⅱ was significantly lower than that of the control group and Group Ⅲ(P<0.05).The eggshell weight of Group Ⅰ and Group Ⅲ was significantly higher than that of the control group(P<0.05).On day 28 of the trial,the expression of eggshell gland plasma membrane calcium ATPase 1b(PMCA 1b)in all treatment groups was significantly higher than that of the control group(P<0.05),and the content of osteocalcin and 25-hydroxyvitamin D3[25-(OH)-D3]in all treatment groups was significantly higher than that of the control group(P<0.05).On day 56 of the trial,the expression of eggshell gland PMCA 1b in Group Ⅱ was significantly higher than that of the other groups(P<0.05),and the content of osteocalcin and 25-(OH)-D3 in Group Ⅱ was lower than that of the other groups(P<0.05).On days 28 and 56 of the trial,the expression of eggshell gland calcium-binding protein(CaBP-D28k)in all treatment groups was significantly higher than that of the control group(P<0.05).On day 28 of the trial,the content of serum interleukin-1β(IL-1β),interleukin-2(IL-2),interleukin-6(IL-6),and tumor necrosis factor-β(TNF-β)in all treatment groups was significantly higher than that of the control group(P<0.05),and the serum total superoxide dismutase(T-SOD)activity of Group Ⅲ was significantly higher than that of the control group(P<0.05).On day 56 of the trial,the serum IL-1β content of Group Ⅰ was significantly higher than that of the control group(P<0.05),the serum IL-1β,IL-2,and TNF-β content of Group Ⅱ was significantly lower than that of the control group(P<0.05),and the IL-6 content was significantly higher than that of the control group(P<0.05).The serum glutathione(GSH)content of Groups Ⅱ and Ⅲ was significantly higher than that of the control group(P<0.05),and the serum malondialdehyde(MDA)content of all treatment groups was significantly lower than that of the control group(P<0.05).The study indicates that the addition of ginseng crude extract to the diet can effectively improve the egg quality,regulate calcium and phosphorus metabolism in hens,and reduce inflammatory responses.Considering the comprehensive effects,the combination of ginseng crude extract with schizophyllan is more optimal.
国家科技期刊平台
NSTL
万方数据
