Effects of mulberry leaf DNJ on growth performance,organ index,antioxidant capacity,and intestinal barrier function of meat rabbits
This study aimed to explore the effects of 1-deoxynojirimycin(DNJ)derived from mulberry leaves on the growth performance,organ index,antioxidant capacity,intestinal barrier function,and intestinal inflammatory factors in New Zealand white rabbits.A total of 36 healthy New Zealand white rabbits,aged 45 days and with similar body weight,were randomly divided into three groups,with 12 replicates per group and one rabbit per replicate.The group T0(control group)was fed a basic diet,the group T1 was fed a basic diet supplemented with 1.4 g/kg mulberry leaf DNJ,and the group T2 was fed a basic diet supplemented with 2.8 g/kg mulberry leaf DNJ.The pre-test period was one week and the formal test period was four weeks.The results showed that compared to group T0,the average daily weight gain was significantly decreased in group T2(P<0.05).The content of serum malondialdehyde(MDA)was significantly decreased in groups T1 and T2(P<0.05),and the content of serum glutathione(GSH)was significantly increased in group T2(P<0.05).The activity of serum glutathione peroxidase(GSH-Px)was significantly increased in group T1(P<0.05).Compared to group T0,the levels of serum immunoglobulin M(IgM)and immunoglobulin A(IgA)in rabbits were significantly increased in group T2(P<0.05).The expression levels of intestinal jejunum junctional adhesion molecule 2(JAM2),junctional adhesion molecule 3(JAM3),mucin4 and mucin6 genes were significantly increased in groups T1 and T2(P<0.05),while the gene expression levels of Toll-like receptor2(TLR2)and Toll-like receptor4(TLR4)were significantly decreased(P<0.05).The gene expression levels of transmembrane proteins Claudin1 and Claudin2 were significantly increased in group T2(P<0.05).The gene expression level of Occluding in the jejunum of rabbits in group T2 was significantly higher than that in group T1(P<0.05).Compared with the group T0,the levels of inflammatory factors tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and C-reactive protein(CRP)in the jejunum of rabbits were significantly reduced in groups T1 and T2(P<0.05).The study indicates that supplementation of 2.8 g/kg mulberry leaf DNJ can inhibit the growth performance of New Zealand white rabbits.Dietary supplementation of 1.4 or 2.8 g/kg mulberry leaf DNJ can enhance the immune performance,antioxidant capacity and intestinal barrier function of New Zealand white rabbits.
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