1株牛源非解乳糖链球菌的分离鉴定及其益生特性的研究
Study of isolation and identification of a Streptococcus alactolyticus of bovine origin and its probiotic properties
魏欣 1庞小童 1聂存喜 1张文举 1尹君亮 2白雪 1陈宁 2吴妍妍1
作者信息
- 1. 石河子大学动物科技学院,新疆 石河子 832003
- 2. 新疆农垦科学院,新疆 石河子 832000
- 折叠
摘要
试验旨在从新生弗荷F1(♂弗莱维赫牛×♀荷斯坦)的不同肠段黏膜中筛选肠道定植菌株并研究其益生特性.试验采用16S rRNA基因测序分析方法对分离菌株进行种属鉴定,并对分离鉴定的乳酸菌株进行体外益生功能的研究,综合评价分离菌株的特性.结果显示,从弗荷F1的不同肠段黏膜中分离出1株乳酸菌候选菌株,经16S rRNA测序鉴定,为非解乳糖链球菌,命名为F2.该菌株对大肠杆菌、金黄色葡萄球菌和沙门氏菌具有潜在的抑菌能力,该菌株具有较高的疏水性、自聚集性、耐酸性、胆汁耐受性和抗生素敏感性.研究表明,从牛源肠道黏膜中分离出的乳酸菌可能具有成为益生菌的潜力.
Abstract
The experiment aimed to screen for gut colonizing bacterial strains from different intestinal mucosal segments of the Ferhe F1cow(♂Fleckvieh×♀Holstein)and to study their probiotic properties.The experiment used 16S rRNA gene sequencing analysis to identify the species of isolated strains and conducted in vitro probiotic function research on the isolated and identified lactic acid bacterial strains to comprehensively evaluate the characteristics of the isolated strains.The results showed that one lactic acid bacterial candidate strain was isolated from the different intestinal mucosal segments of the Ferhe F1cow identified by 16S rRNA sequencing,it was a Streptococcus alactolyticus,named F2.This strain had potential inhibitory effects on Escherichia coli,Staphylococcus aureus,and Salmonella.The strain has high hydrophobicity,auto-aggregation,acid tolerance,bile tolerance,and antibiotic sensitivity.The study indicates that lactic acid bacteria isolated from bovine origin of intestinal mucosa may have the potential to become probiotics.
关键词
牛源/乳酸菌/分离鉴定/益生特性Key words
bovine origin/lactic acid bacteria/isolation and identification/probiotic properties引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据