Effect of compounding Cinnamomum cassia essential oil and Litsea cubeba essential oil on biofilm of Salmonella pullorum
The study aimed to investigate the synergistic antibacterial effects of Cinnamomum cassia essential oil(CEO)and Litsea cubeba essential oil(LEO)against Salmonella(S.pullorum SP1)and their effects on biofilm.The minimum inhibitory concentration(MIC)and fractional inhibitory concentration index(FICI)were determined to evaluate the antibacterial activity of CEO and LEO against SP1.Changes in nucleic acid and protein leakage and reactive oxygen species(ROS)production were measured to explore the antibacterial mechanism.The effects of CEO and LEO on SP1 biofilm were evaluated by assessing biofilm inhibition,removal ability,and extracellular polymeric substance(EPS)content.The results showed that the MIC of CEO against SP1 was 0.39 g/L,while the MIC of LEO was 1.56 g/L.The FICI of the combination was 0.5.The combination of essential oils disrupted the cell integrity of SP1,leading to significant increases in extracellular nucleic acid,protein,and ROS levels(P<0.05).The combination of essential oils inhibited biofilm formation(P<0.05)and significantly reduced biofilm biomass after 24 hours(P<0.05).The extracellular polysaccharide content and aggregation ability of SP1 were significantly decreased in the combination group and 2×combination group(P<0.05).The study indicates that CEO and LEO have inhibitory effects against SP1,and the combination of essential oils can disrupt the structural integrity of Salmonella,induce oxidative damage,inhibit biofilm formation,reduce extracellular polysaccharide content,and decrease bacterial aggregation ability,thereby achieving inhibitory effects against Salmonella.
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